Dual effects of phytoestrogens result in u-shaped dose-response curves Almstrup, Kristian Fernández, Mariana F. Petersen, Jørgen Olea Serrano, Nicolás Skakkebæk, Niels Leffers, Henrik Aromatase inhibitors Endocrine disruptors Estrogenicity Phytoestrogens U-shaped dose-response curves 'Reproduced with permission from Environmental Health Perspectives' Endocrine disruptors can affect the endocrine system without directly interacting with receptors, for example, by interfering with the synthesis or metabolism of steroid hormones. The aromatase that converts testosterone to 17β-estradiol is a possible target. In this paper we describe an assay that simultaneously detects aromatase inhibition and estrogenicity. The principle is similar to that of other MCF-7 estrogenicity assays, but with a fixed amount of testosterone added. The endogenous aromatase activity in MCF-7 cells converts some of the testosterone to 17β-estradiol, which is assayed by quantifying differences in the expression level of the estrogen-induced pS2 mRNA. Potential aromatase inhibitors can be identified by a dose-dependent reduction in the pS2 mRNA expression level after exposure to testosterone and the test compound. Using this assay, we have investigated several compounds, including synthetic chemicals and phytoestrogens, for aromatase inhibition. The phytoestrogens, except genistein, were aromatase inhibitors at low concentrations (< 1 μM) but estrogenic at higher concentrations (≥ 1 μM), resulting in U-shaped dose–response curves. None of the tested synthetic chemicals were aromatase inhibitors. The lowdose aromatase inhibition distinguished phytoestrogens from other estrogenic compounds and may partly explain reports about antiestrogenic properties of phytoestrogens. Aromatase inhibition may play an important role in the protective effects of phytoestrogens against breast cancer. 2013-05-07T12:12:46Z 2013-05-07T12:12:46Z 2002 info:eu-repo/semantics/article Almstrup, K.; et al. Dual effects of phytoestrogens result in u-shaped dose-response curves. Environmental Health Perspectives, 110(8): 743-748 (2002). [http://hdl.handle.net/10481/24960] 0091-6765 1552-9924 PMCID: PMC1240943 http://hdl.handle.net/10481/24960 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License National Institute of Environmental Health Sciences