BI-OBJECTIVE OPTIMISATION OF THE ENZYMATIC HYDROLYSIS OF PORCINE BLOOD PROTEIN
Identificadores
URI: https://hdl.handle.net/10481/88521Metadatos
Mostrar el registro completo del ítemAutor
Pérez Gálvez, Antonio Raúl; Almecija Rodríguez, María Carmen; Espejo Carpio, Francisco Javier; Guadix Escobar, Antonio María; Guadix Escobar, Emilia MaríaEditorial
ELSEVIER
Materia
Blood meal Enzyme bioreactors Modelling Bi-objective optimisation Proteolysis Response surface methodology
Fecha
2011-02Referencia bibliográfica
Raúl Pérez-Gálvez, M.Carmen Almecija, F.Javier Espejo, Emilia M. Guadix, Antonio Guadix (2011). Biochemical Engineering Journal 53: 305-310
Patrocinador
Ministerio de Ciencia e Innovación ( CTQ2008-02978)Resumen
Protein from porcine blood meal was hydrolysed with Alcalase to obtain a final revalorised product suitable, for example, to take part in the composition of an organic fertiliser. Three experimental factors of the reaction (pH, temperature and enzyme-substrate ratio) were optimised by means of a statistically designed experiment and response surface methodology. The goal of the optimisation problem was to maximise both the degree of hydrolysis and solubilisation of the substrate, obtaining a maximum degree of hydrolysis (28.89%) with pH 6.24, 54.2 °C and enzyme-substrate ratio of 10%. Regarding the content of suspended solids, its minimum value (30.29% related to the initial weight of blood meal) was attained at pH 7.5, 59.8 °C and enzyme-substrate ratio of 10%. The controversial effects of pH and temperature on the substrate solubilisation and the final degree of hydrolysis, suggested employing a multiobjective optimisation technique. A Pareto Front was generated in order to find a set of intermediate solutions which satisfied both objectives in an adequate degree.