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gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking
dc.contributor.author | Neubrand, Veronika Elisabeth | |
dc.contributor.author | Will, Rainer D | |
dc.contributor.author | Möbius, Wiebke | |
dc.contributor.author | Poustka, Annemarie | |
dc.contributor.author | Wiemann, Stefan | |
dc.contributor.author | Schu, Peter | |
dc.contributor.author | Dotti, Carlos G | |
dc.contributor.author | Pepperkok, Rainer | |
dc.contributor.author | Simpson, Jeremy C | |
dc.date.accessioned | 2024-01-03T11:30:56Z | |
dc.date.available | 2024-01-03T11:30:56Z | |
dc.date.issued | 2005 | |
dc.identifier.citation | EMBO J 24, 1122–1133 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10481/86543 | |
dc.description.abstract | A novel peripheral membrane protein (2c18) that interacts directly with the gamma ‘ear’ domain of the adaptor protein complex 1 (AP-1) in vitro and in vivo is described. Ultrastructural analysis demonstrates a colocalization of 2c18 and c1-adaptin at the trans-Golgi network (TGN) and on vesicular profiles. Overexpression of 2c18 increases the fraction of membrane-bound c1-adaptin and inhibits its release from membranes in response to brefeldin A. Knockdown of 2c18 reduces the steady-state levels of c1-adaptin on membranes. Overexpression or downregulation of 2c18 leads to an increased secretion of the lysosomal hydrolase cathepsin D, which is sorted by the mannose-6-phosphate receptor at the TGN, which itself involves AP-1 function for trafficking between the TGN and endosomes. This suggests that the direct interaction of 2c18 and c1-adaptin is crucial for membrane association and thus the function of the AP-1 complex in living cells. We propose to name this protein c-BAR. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | EMBO Press | es_ES |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | es_ES |
dc.subject | AP-1 | es_ES |
dc.subject | Gamma-adaptin | es_ES |
dc.title | gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.doi | 10.1038/ sj.emboj.7600600 | |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |