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Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline

dc.contributor.authorRybakowska, Paulina
dc.contributor.authorMartorell Marugán, Jordi 
dc.contributor.authorAlarcón Riquelme, Marta Eugenia 
dc.contributor.authorMarañón Lizana, Concepción
dc.date.accessioned2023-10-25T10:23:03Z
dc.date.available2023-10-25T10:23:03Z
dc.date.issued2022-12-16
dc.identifier.citationRybakowska P. et al. Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline. STAR Protocols 3, 4, 101697. [https://doi.org/10.1016/j.xpro.2022.101697]es_ES
dc.identifier.urihttps://hdl.handle.net/10481/85242
dc.descriptionSupport has been received (PI: M.E.A.) from the IMI2-JU project GA No 831434 (3TR) and IMI-JU project GA No 115565 (PRECISESADS). P.R. has received support from EMBO (7966) and from Consejería de Salud de Junta de Andalucía (EF-0091-2018). C.M. acknowledges funding from Programa Nicolas Monardes (C2-0002-2019). J.M.M. is funded by European Union-NextGenerationEU, Ministry of Universities (Spain’s Government) and the Recovery, Transformation and Resilience Plan. These results form a part of the P.R. PhD thesis in Biomedicine at the University of Granada. We are grateful to Olivia Santiago and Jose Diaz Cuéllar for technical support as a Core facility in Genyo research center. Also, we would like to express our gratitude to the donors. The figures in this paper were created with BioRender.comes_ES
dc.description.abstractMass cytometry (MC) is a powerful large-scale immune monitoring technology. To maximize MC data quality, we present a protocol for whole blood analysis together with an R package, Cyto Quality Pipeline (CytoQP), which minimizes the experimental artifacts and batch effects to ensure data reproducibility. We describe the steps to stimulate, fix, and freeze blood samples before acquisition to make them suitable for retrospective studies. We then detail the use of bar-coding and reference samples to facilitate multicenter and multi-batch experiments.For complete details on the use and execution of this protocol, please refer to Rybakowska et al. (2021a) and (2021b).es_ES
dc.description.sponsorshipIMI2-JU project GA 831434es_ES
dc.description.sponsorshipIMI-JUproject GA 115565es_ES
dc.description.sponsorshipEuropean Molecular Biology Organization (EMBO) 7966es_ES
dc.description.sponsorshipJunta de Andalucía EF-0091-2018es_ES
dc.description.sponsorshipPrograma Nicolás Monardes C2-0002-2019es_ES
dc.description.sponsorshipEuropean Union-NextGenerationEUes_ES
dc.description.sponsorshipMinistry of Universities (Spain's Government) and the Recovery, Transformation and Resilience Planes_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleProtocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipelinees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.1016/j.xpro.2022.101697
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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