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Protection of Catalytic Cofactors by Polypeptides as a Driver for the Emergence of Primordial Enzymes
dc.contributor.author | Gutiérrez Rus, Luis Ignacio | |
dc.contributor.author | Gámiz Arco, María Gloria | |
dc.contributor.author | Gavira Gallardo, José Antonio | |
dc.contributor.author | Risso, Valeria Alejandra | |
dc.contributor.author | Sánchez Ruiz, José Manuel | |
dc.date.accessioned | 2023-07-27T08:29:08Z | |
dc.date.available | 2023-07-27T08:29:08Z | |
dc.date.issued | 2023-05-26 | |
dc.identifier.citation | Luis I Gutierrez-Rus and others, Protection of Catalytic Cofactors by Polypeptides as a Driver for the Emergence of Primordial Enzymes, Molecular Biology and Evolution, Volume 40, Issue 6, June 2023, msad126, [https://doi.org/10.1093/molbev/msad126] | es_ES |
dc.identifier.uri | https://hdl.handle.net/10481/84039 | |
dc.description.abstract | Enzymes catalyze the chemical reactions of life. For nearly half of known enzymes, catalysis requires the binding of small molecules known as cofactors. Polypeptide-cofactor complexes likely formed at a primordial stage and became starting points for the evolution of many efficient enzymes. Yet, evolution has no foresight so the driver for the primordial complex formation is unknown. Here, we use a resurrected ancestral TIM-barrel protein to identify one potential driver. Heme binding at a flexible region of the ancestral structure yields a peroxidation catalyst with enhanced efficiency when compared to free heme. This enhancement, however, does not arise from proteinmediated promotion of catalysis. Rather, it reflects the protection of bound heme from common degradation processes and a resulting longer lifetime and higher effective concentration for the catalyst. Protection of catalytic cofactors by polypeptides emerges as a general mechanism to enhance catalysis and may have plausibly benefited primordial polypeptide-cofactor associations. | es_ES |
dc.description.sponsorship | Human Frontier Science Program grant RGP0041/2017 | es_ES |
dc.description.sponsorship | National Science Foundation grant 2032315 | es_ES |
dc.description.sponsorship | Department of Defense grant MURI W911NF-16-1-0372 | es_ES |
dc.description.sponsorship | National Institutes of Health grant R01AR069137 | es_ES |
dc.description.sponsorship | Spanish Ministry of Science and Innovation/ FEDER Funds grant PID2021-124534OB-100 | es_ES |
dc.description.sponsorship | Grant PID2020-116261GB-I00 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Oxford academy | es_ES |
dc.rights | Atribución-NoComercial 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.title | Protection of Catalytic Cofactors by Polypeptides as a Driver for the Emergence of Primordial Enzymes | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.doi | 10.1093/molbev/msad126 | |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |