Bioinspired Mineralization of Type I Collagen Fibrils with Apatite in Presence of Citrate and Europium Ions
Metadatos
Mostrar el registro completo del ítemAutor
Gómez Morales, Jaime; Fernández Penas, Raquel; Verdugo-Escamilla, Cristóbal; Degli Esposti, Lorenzo; Oltolina, Francesca; Prat, María; Iafisco, Michele; Fernández Sánchez, Jorge FernandoEditorial
MDPI
Materia
Collagen mineralization Apatite Amorphous calcium phosphate Citrate Europium Luminescence Cytocompatibility
Fecha
2018-12-25Referencia bibliográfica
Gómez Morales, J. [et al.]. Bioinspired Mineralization of Type I Collagen Fibrils with Apatite in Presence of Citrate and Europium Ions. Crystals 2019, 9, 13; doi:10.3390/cryst9010013.
Patrocinador
This research was funded by Spanish MINEICO and co–funded by FEDER (grant number MAT2014–60533–R). C. V.–E. acknowledges the Spanish MINEICO for his contract PTA2015–11103–I. M.I. acknowledges support by the Italian National Research Program–National Research Council (PNRCNR) Aging Program 2012–2014. The Excellence Network of Crystallography and Crystallization “Factoría de Cristalización” FIS2015–71928–REDC funded by Spanish MINEICO is also acknowledged.Resumen
Synthetic nanostructured hybrid composites based on collagen and nanocrystalline apatites
are interesting materials for the generation of scaffolds for bone tissue engineering. In this work,
mineralized collagen fibrils were prepared in the presence of citrate and Eu3+. Citrate is an
indispensable and essential structural/functional component of bone. Eu3+ endows the mineralized
fibrils of the necessary luminescent features to be potentially employed as a diagnostic tool in
biomedical applications. The assembly and mineralization of collagen were performed by the
neutralization method, which consists in adding dropwise a Ca(OH)2 solution to a H3PO4 solution
containing the dispersed type I collagen until neutralization. In the absence of citrate, the resultant
collagen fibrils were mineralized with nanocrystalline apatites. When citrate was added in the
titrant solution in a Citrate/Ca molar ratio of 2 or 1, it acted as an inhibitor of the transformation of
amorphous calcium phosphate (ACP) to nanocrystalline apatite. The addition of Eu3+ and citrate
in the same titrant solution lead to the formation of Eu3+–doped citrate–coated ACP/collagen
fibrils. Interestingly, the relative luminescent intensity and luminescence lifetime of this latter
composite were superior to those of Eu3+–doped apatite/collagen prepared in absence of citrate.
The cytocompatibility tests, evaluated by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide) (MTT) colorimetric assay in a dose–dependent manner on GTL–16 human gastric carcinoma
cells, on MG–63 human osteosarcoma cells and on the m17.ASC, a spontaneously immortalized
mouse mesenchymal stem cell clone from subcutaneous adipose tissue, show that, in general, all
samples are highly cytocompatible.