Mostrar el registro sencillo del ítem
Photophysics of a Live-Cell-Marker, Red Silicon-Substituted Xanthene Dye
dc.contributor.author | Crovetto González, Luis | |
dc.contributor.author | Orte Gutiérrez, Ángel | |
dc.contributor.author | Paredes, José M. | |
dc.contributor.author | Resa, Sandra | |
dc.contributor.author | Valverde Pozo, Javier | |
dc.contributor.author | Castello, Fabio | |
dc.contributor.author | Miguel, Delia | |
dc.contributor.author | Cuerva, Juan M. | |
dc.contributor.author | Talavera Rodríguez, Eva María | |
dc.contributor.author | Álvarez Pez, José María | |
dc.date.accessioned | 2019-03-07T11:15:08Z | |
dc.date.available | 2019-03-07T11:15:08Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Crovetto, L. [et al.] Photophysics of a Live-Cell-Marker, Red Silicon-Substituted Xanthene Dye. J. Phys. Chem. A 2015, 119, 44, 10854-10862 | es_ES |
dc.identifier.uri | https://pubs.acs.org/doi/10.1021/acs.jpca.5b07898 | |
dc.identifier.uri | http://hdl.handle.net/10481/54892 | |
dc.description | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry A, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.jpca.5b07898. | es_ES |
dc.description.abstract | Dyes with near-red emission are of great interest because of their undoubted advantages for use as probes in living cells. In-depth knowledge of their photophysics is essential for employment of such dyes. In this article, the photophysical behavior of a new siliconsubstituted xanthene, 7-hydroxy-5,5-dimethyl-10-(o-tolyl)dibenzo[b,e]silin-3(5H)-one (2-Me TM), was explored using absorption, steady-state, and time-resolved fluorescence. First, the near-neutral pH, ground state acidity constant of the dye, pK*N-A, was determined by both absorbance and steady-state fluorescence methods in the presence of very low buffer concentrations. Next, we determined whether the addition of phosphate buffer promoted the excited-state proton exchange reaction between the neutral and anion form of 2-Me TM in aqueous solutions at near-neutral pH. For this analysis, both the steady-state fluorescence method and time-resolved emission spectroscopy (TRES) were employed. The TRES experiments demonstrated a remarkably favored conversion of the neutral form to the anion form. Then, fluorescence decay traces recorded as a function of buffer concentration and pH were globally analyzed to determine the values of the excited-state rate constants. The revealed kinetic parameters were consistent with the TRES results, exhibiting a higher rate constant for deprotonation than for protonation, which implies an unusual low value of the excited-state acidity constant pK*N-A and therefore an enhanced photoacid behavior of the neutral form. Finally, we determined whether 2-Me TM could be used as a sensor inside live cells by measuring the intensity profile of the probe in different cellular compartments of HeLa 229 cells. | es_ES |
dc.description.sponsorship | This research was funded by MINECO (project CTQ2014-56370-R), and MINECO (project CTQ2014-53598). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.title | Photophysics of a Live-Cell-Marker, Red Silicon-Substituted Xanthene Dye | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.doi | 10.1021/acs.jpca.5b07898 |