Bacterial density rather than diversity correlates with hatching success across different avian species
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AuthorPeralta-Sánchez, Juan Manuel; Martín-Platero, Antonio Manuel; Wegener-Parfrey, Laura; Martínez Bueno, Manuel; Rodríguez-Ruano, Sonia; Navas-Molina, José Antonio; Vázquez-Baeza, Yoshiki; Martín-Gálvez, David; Martín-Vivaldi, Manuel; Ibáñez-Álamo, Juan Diego; Knight, Rob; Soler, Juan José
Oxford University Press
ARISAAvian communityBacterial communityBacterial densityComparative analysisEggshellsHatching successHigh-throughput sequencingIllumina HiSeqPhylogenetic General Least Square
Peralta-Sánchez, Juan Manuel; et. al. Bacterial density rather than diversity correlates with hatching success across different avian species. FEMS Microbiology Ecology, 2018, Vol. 94, No. 3 fiy022 [http://hdl.handle.net/10481/53913]
SponsorshipFunding was provided by Ministerio de Educación y Ciencia and European founds (FEDER) [CGL2007-61251, CGL2010-19233-C03- 01, CGL2010-19233-C03-03]. JMPS was funded by Ministerio de Educación and Consejería de Innovación, Ciencia y Empresa under International Excellence Campus Program, University of Granada (CEI Granada 2009). RK was supported in part by the Howard Hughes Medical Institute. The Earth Microbiome Project was supported in part by the John Templeton Foundation and the W.M. Keck Foundation.
Bacterial communities within avian nests are considered an important determinant of egg viability, potentially selecting for traits that confer embryos with protection against trans-shell infection. A high bacterial density on the eggshell increases hatching failure, whether this effect could be due to changes in bacterial community or just a general increase in bacterial density. We explored this idea using intra- and interspecific comparisons of the relationship between hatching success and eggshell bacteria characterized by culture and molecular techniques (fingerprinting and high-throughput sequencing). We collected information for 152 nests belonging to 17 bird species. Hatching failures occurred more frequently in nests with higher density of aerobic mesophilic bacteria on their eggshells. Bacterial community was also related to hatching success, but only when minority bacterial operational taxonomic units were considered. These findings support the hypothesis that bacterial density is a selective agent of embryo viability, and hence a proxy of hatching failure only within species. Although different avian species hold different bacterial densities or assemblages on their eggs, the association between bacteria and hatching success was similar for different species. This result suggests that interspecific differences in antibacterial defenses are responsible for keeping the hatching success at similar levels in different species.