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dc.contributor.authorFernández-Caballero, José Ángeles_ES
dc.contributor.authorChueca-Porcuna, Nataliaes_ES
dc.contributor.authorÁlvarez Alday, Martaes_ES
dc.contributor.authorMerida, María Doloreses_ES
dc.contributor.authorLópez, Josefaes_ES
dc.contributor.authorSánchez, José Antonioes_ES
dc.contributor.authorVinuesa García, Davides_ES
dc.contributor.authorMartínez Pérez, María Ángeles es_ES
dc.contributor.authorHernández-Quero, Josées_ES
dc.contributor.authorGarcía García, Federicoes_ES
dc.identifier.citationFernández-Caballero, J. A.; et al. Usefulness of Integrase resistance testing in proviral HIV-1 DNA in patients with Raltegravir prior failure. BMC Infectious Diseases, 16: 197 (2016). []es_ES
dc.description.abstractBackground: In our study, we have hypothesized that proviral DNA may show the history of mutations that emerged at previous failures to a Raltegravir containing regimen, in patients who are currently undetectable and candidates to simplification to a Dolutegravir containing regimen, in order to decide on once a day or twice a day dosing. Methods: We have performed a pilot, observational, retrospective, non interventional study, including 7 patients infected by HIV-1, all with a history of previous failure to a RAL containing regimen, that were successfully salvaged and had reached viral suppression. A genotypic viral Integrase region study was available for each patient at the moment of RAL failure. After an average (IQR) time of 48 months (29–53) Integrase resistance mutations in proviral DNA were studied. Results: All the patients were infected by HIV-1 B subtypes, with a mean age of 55 (range 43 to 56), originating from Spain, and 4 were women. Median viral load (log) and CD4 count at the moment of the study on proviral DNA was of 1.3 log cp/ml (range 0–1.47) and 765.5 cells/μL (range; 436.75–1023.75). The median time (IQR) between previous failure to RAL and the study on proviral DNA was 48 (29–53) months. At Raltegravir failure, N155H was detected in four patients, and other secondary mutations were detected in five patients (71.4 %). In proviral DNA, N155H was detected by population sequencing in three patients (42.8 %), and UDS demonstrated a 9.77 % relative abundance of N155H in the remaining patient. Sanger sequencing correctly identified all the secondary mutations. Conclusion: This is a pilot study that demonstrates the possibility of properly identifying N155H and some secondary mutations 29–53 months after failure.en_EN
dc.description.sponsorshipThis study was funded by Fondo de Investigación Sanitaria (PI12/01053, PI15/00713), RD12/0017/006 (Plan Nacional de I + D + I, Fondo Europeo de Desarrollo Regional-FEDER).en_EN
dc.publisherBiomed Centrales_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 License
dc.subjectProviral DNAen_EN
dc.titleUsefulness of Integrase resistance testing in proviral HIV-1 DNA in patients with Raltegravir prior failureen_EN

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