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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/49236

Title: Development of polymorphic microsatellite markers for the Killarney Fern (Vandenboschia speciosa, Hymenophyllaceae)
Authors: García-López, María del Carmen
Ben-Menni Schuler, Samira
López Flores, Inmaculada
Nieto-Lugilde, Marta
Terrón-Camero, Laura
Suárez Santiago, Víctor
Issue Date: 4-Nov-2015
Abstract: Premise of the study: We characterize 10 microsatellite loci in the endangered fern Vandenboschia speciosa (Hymenophyllaceae), enabling studies on the genetic population structure of this Macaronesian-European species using DNA hypervariable markers. Methods and Results: Ten primer sets were developed and tested on 47 individuals in a total of two Iberian populations of V. speciosa. The primers amplified di- and hexanucelotide repeats. The number of alleles ranged from two to eight, and the expected heterozygosity ranged from 0.107 to 0.807 among the populations analyzed. Conclusions: The 10 microsatellite markers developed will be useful in characterizing the genetic diversity of V. speciosa and understanding its population structure (including the possible structure between sporophyte and gametophyte phases) and biogeographic history, and will provide important genetic data for the conservation of this species.
Sponsorship: This study was supported by the Regional Andalusian Government (project P10-RNM-6198). C.G.L. was funded by a postdoctoral grant from the Regional Andalusian Government. S.B.S. and L.T.C. were funded by a Beca-Iniciación a la Investigación grant from the Universidad de Granada during 2012–2013 and 2014–2015, respectively. I.M.A. was funded by a Beca-Colaboración grant (Spanish Government).
Publisher: Botanical Society of America; Wiley
Keywords: Endangered fern
Hymenophyllaceae
Microsatellite markers
Population genetics
Vandenboschia speciosa
URI: http://hdl.handle.net/10481/49236
ISSN: 2168-0450
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: García-López, M.C.; et al. Development of polymorphic microsatellite markers for the Killarney Fern (Vandenboschia speciosa, Hymenophyllaceae). Applications in Plant Sciences, 3(11): 1500067 (2015). [http://hdl.handle.net/10481/49236]
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