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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/47518

Title: McCLEC, a robust and stable enzymatic based microreactor platform
Authors: Conejero-Muriel, Mayte
Rodríguez-Ruiz, Isaac
Martínez-Ruiz, Sergio
Llobera, Andreu
Gavira Gallardo, José Antonio
Issue Date: 20-Aug-2015
Abstract: A microfluidic chip for cross-linked enzyme crystals (McCLEC) is presented and demonstrated to be a stable, reusable and robust biocatalyst-based device with very promising biotechnological applications. The cost-effective microfluidic platform allows in situ crystallization, cross-linking and enzymatic reaction assays on a single device. A large number of enzymatic reuses of the McCLEC platform were achieved and a comparative analysis is shown illustrating the efficiency of the process and its storage stability for more than one year.
Sponsorship: This work has been partly funded by the MICINN (Spain) projects BIO2010-16800 (JAG) and BIO2012-34937 (SMR), the European Commission (Contract No. 317916) under the LiPhos project (AL and IRR), “Factoría Española de Cristalización” Consolider-Ingenio 2010 (JAG and MCM) and EDRF Funds (JAG and AL). MCM thanks the Consejo Superior de Investigaciones Científicas (CSIC, Spain) for a JAE predoc fellowship.
Publisher: Royal Society of Chemistry
Keywords: Microfluidic
Crystallization
Device
McCLEC
Enzyme Assay
URI: http://hdl.handle.net/10481/47518
ISSN: 1473-0197
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Conejero-Muriel, M.; et al. McCLEC, a robust and stable enzymatic based microreactor platform. Lab on a Chip, 15: 4083-4089 (2015). [http://hdl.handle.net/10481/47518]
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