McCLEC, a robust and stable enzymatic based microreactor platform
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AuthorConejero-Muriel, Mayte; Rodríguez-Ruiz, Isaac; Martínez-Ruiz, Sergio; Llobera, Andreu; Gavira Gallardo, José Antonio
Royal Society of Chemistry
Conejero-Muriel, M.; et al. McCLEC, a robust and stable enzymatic based microreactor platform. Lab on a Chip, 15: 4083-4089 (2015). [http://hdl.handle.net/10481/47518]
SponsorshipThis work has been partly funded by the MICINN (Spain) projects BIO2010-16800 (JAG) and BIO2012-34937 (SMR), the European Commission (Contract No. 317916) under the LiPhos project (AL and IRR), “Factoría Española de Cristalización” Consolider-Ingenio 2010 (JAG and MCM) and EDRF Funds (JAG and AL). MCM thanks the Consejo Superior de Investigaciones Científicas (CSIC, Spain) for a JAE predoc fellowship.
A microfluidic chip for cross-linked enzyme crystals (McCLEC) is presented and demonstrated to be a stable, reusable and robust biocatalyst-based device with very promising biotechnological applications. The cost-effective microfluidic platform allows in situ crystallization, cross-linking and enzymatic reaction assays on a single device. A large number of enzymatic reuses of the McCLEC platform were achieved and a comparative analysis is shown illustrating the efficiency of the process and its storage stability for more than one year.