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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/47248

Title: Production and characterization of functional recombinant hybrid heteropolymers of camel hepcidin and human ferritin H and L chains
Authors: Boumaiza, Mohamed
Carmona Rodríguez-Acosta, Fernando
Poli, Maura
Asperti, Michela
Gianoncelli, Alessandra
Bertuzzi, Michela
Ruzzenenti, Paola
Arosio, Paolo
Marzouki, Mohamed
Issue Date: Jan-2017
Abstract: Hepcidin is a liver-synthesized hormone that plays a central role in the regulation of systemic iron homeostasis. To produce a new tool for its functional properties the cDNA coding for camel hepcidin-25 was cloned at the 5’end of human FTH sequence into the pASK-IBA43plus vector for expression in Escherichia coli. The recombinant fusion hepcidin–ferritin-H subunit was isolated as an insoluble iron-containing protein. When alone it did not refold in a 24-mer ferritin molecule, but it did when renatured together with H- or L-ferritin chains. We obtained stable ferritin shells exposing about 4 hepcidin peptides per 24-mer shell. The molecules were then reduced and re-oxidized in a controlled manner to allow the formation of the proper hepcidin disulfide bridges. The functionality of the exposed hepcidin was confirmed by its ability to specifically bind the mouse macrophage cell line J774 that express ferroportin and to promote ferroportin degradation. This chimeric protein may be useful for studying the hepcidin–ferroportin interaction in cells and also as drug-delivery agent.
Sponsorship: This work is partially financed by the Laboratory of Protein Engineering and Bioactive Molecules (LIP-MB) and the Doctoral School of the National Institute of Applied Sciences and Technology (INSAT-Tunis) – University of Carthage.
Publisher: Oxford University Press
Description: This article has been accepted for publication in Protein Engineering design and Selection Published by Oxford University Press.
Keywords: Hepcidin
Ferritin
Heteropolymers
Camel hepcidin
Human ferritin H and L chains
J774 cells
URI: http://hdl.handle.net/10481/47248
ISSN: 1741-0126
1741-0134
Citation: Boumaiza, M.; et al. Production and characterization of functional recombinant hybrid heteropolymers of camel hepcidin and human ferritin H and L chains. Protein Engineering, Design and Selection 30(2): 77-84 (2017). [http://hdl.handle.net/10481/47248]
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