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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/47231

Title: Chemically and Biologically Harmless versus Harmful Ferritin/Copper–Metallothionein Couples
Authors: Carmona Rodríguez-Acosta, Fernando
Mendoza, Daniela
Kord, Scheghajegh
Asperti, Michela
Arosio, Paolo
Atrian, Silvia
Capdevila, Mercé
Domínguez Vera, José Manuel
Issue Date: 2014
Abstract: The simultaneous measurement of the decrease of available FeII ions and the increase of available FeIII ions allowed the analysis of the ferroxidase activity of two distinct apoferritins. Although recombinant human apoferritin (HuFtH) rapidly oxidizes FeII to FeIII, this iron is not properly stored in the ferritin cavity, as otherwise occurs in horse-spleen H/L-apoferritin (HsFt; H=heavy subunit, L=light subunit). Iron storage in these apoferritins was also studied in the presence of two copper-loaded mammalian metallothioneins (MT2 and MT3), a scenario that occurs in different brain-cell types. For HuFtH, unstored FeIII ions trigger the oxidation of Cu–MT2 with concomitant CuI release. In contrast, there is no reaction with Cu–MT2 in the case of HsFt. Similarly, Cu–MT3 does not react during either HuFtH or HsFt iron reconstitution. Significantly, the combination of ferritin and metallothionein isoforms reported in glia and neuronal cells are precisely those combinations that avoid a harmful release of FeII and CuI ions.
Sponsorship: Work supported by the Spanish MINECO and FEDER funds with grants CTQ2012–32236 to J.M.D.-V., BIO2012–39682-C02–01 to S.A., and BIO2012–39682-C02–02 to M.C. The authors from the Barcelona universities are members of the Grup de Recerca de la Generalitat de Catalunya (refs. 2014SGR-00423). F.C. is grateful to the Spanish MINECO for a FPI Fellowship.
Publisher: Wiley - VCH Verlag GmbH and Co. KGaA
Description: "This is the peer reviewed version of the following article: Carmona Rodríguez-Acosta, F.; et al. Chemically and Biologically Harmless versus Harmful Ferritin/Copper–Metallothionein Couples. Chemistry A European Journal, 21(2): 808-813 (2015), which has been published in final form at http://dx.doi.org/10.1002/chem.201404660. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."
Keywords: Ferritin
Metallothionein
Brain
Iron
Copper
Biological activity
Metabolism
Metalloenzymes
URI: http://hdl.handle.net/10481/47231
ISSN: 1521-3765
Citation: Carmona Rodríguez-Acosta, F.; et al. Chemically and Biologically Harmless versus Harmful Ferritin/Copper–Metallothionein Couples. Chemistry A European Journal, 21(2): 808-813 (2015). [http://hdl.handle.net/10481/47231]
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