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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/47218

Title: Monitoring lactoferrin iron levels by fluorescence resonance energy transfer: A combined chemical and computational study
Authors: Carmona Rodríguez-Acosta, Fernando
Muñoz-Robles, Víctor
Cuesta Martos, Rafael
Gálvez Rodríguez, Natividad
Capdevila, Mercè
Maréchal, Jean-Didier
Domínguez Vera, José Manuel
Issue Date: 2014
Abstract: Three forms of lactoferrin (Lf) that differed in their levels of iron loading (Lf, LfFe, and LfFe2) were simultaneously labeled with the fluorophores AF350 and AF430. All three resulting fluorescent lactoferrins exhibited fluorescence resonance energy transfer (FRET), but they all presented different FRET patterns. Whereas only partial FRET was observed for Lf and LfFe, practically complete FRET was seen for the holo form (LfFe2). For each form of metal-loaded lactoferrin, the AF350–AF430 distance varied depending on the protein conformation, which in turn depended on the level of iron loading. Thus, the FRET patterns of these lactoferrins were found to correlate with their iron loading levels. In order to gain greater insight into the number of fluorophores and the different FRET patterns observed (i.e., their iron levels), a computational analysis was performed. The results highlighted a number of lysines that have the greatest influence on the FRET profile. Moreover, despite the lack of an X-ray structure for any LfFe species, our study also showed that this species presents modified subdomain organization of the N-lobe, which narrows its iron-binding site. Complete domain rearrangement occurs during the LfFe to LfFe2 transition. Finally, as an example of the possible applications of the results of this study, we made use of the FRET fingerprints of these fluorescent lactoferrins to monitor the interaction of lactoferrin with a healthy bacterium, namely Bifidobacterium breve. This latter study demonstrated that lactoferrin supplies iron to this bacterium, and suggested that this process occurs with no protein internalization.
Sponsorship: This work was supported by MINECO and FEDER (projects CTQ2012-32236, CTQ2011-23336, and BIO2012-39682-C02-02) and BIOSEARCH SA. F.C. and V.M.R. are grateful to the Spanish MINECO for FPI fellowships.
Publisher: Springer Verlang
Keywords: FRET
Lactoferrin
Docking
Iron metabolism
Protein-ligand docking
Structural analysis
URI: http://hdl.handle.net/10481/47218
ISSN: 0949-8257
1432-1327
Citation: Carmona Rodríguez-Acosta, F.; et al. Monitoring lactoferrin iron levels by fluorescence resonance energy transfer: A combined chemical and computational study. Journal of Biological Inorganic Chemistry, 19(3): 439-447 (2014). [http://hdl.handle.net/10481/47218]
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