Zinc-Containing Restorations Create Amorphous Biogenic Apatite at the Carious Dentin Interface: A X-Ray Diffraction (XRD) Crystal Lattice Analysis
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AutorToledano Pérez, Manuel; Aguilera, Fátima; López-López, Modesto T.; Osorio Ruiz, Estrella; Toledano-Osorio, Manuel; Osorio Ruiz, Raquel
Microscopy Society of America
Toledano Pérez, M.; et al. Zinc-Containing Restorations Create Amorphous Biogenic Apatite at the Carious Dentin Interface: A X-Ray Diffraction (XRD) Crystal Lattice Analysis. Microscopy and Microanalysis, 22(5): 1034-1046 (2016). [http://hdl.handle.net/10481/46738]
PatrocinadorThis work was supported by grants MINECO/FEDER MAT2014-52036-P and FIS2013-41821-R.
The aim of this research was to assess the ability of amalgam restorations to induce amorphous mineral precipitation at the caries-affected-dentin substrate. Sound and caries-affected dentin surfaces were subjected to both Zn-free and Zn-containing dental amalgam restorations. Specimens were submitted to thermocycling (100,000cy/5 ºC-55 ºC, 3 months). Dentin surfaces were studied by atomic force microscopy (AFM/nano-roughness), X-ray diffraction (XRD2), field emission electron microscopy (FESEM) and energy-dispersive analysis (EDX), for physical and morphological surface characterization. Zn-containing amalgam placement reduced crystallinity, crystallite size and grain size of calcium phosphate crystallites at the dentin surface. Both microstrain and nanoroughness augmented in caries-affected dentin restored with Zn-containing amalgams. Caries-affected dentin showed the shortest mineral crystallites (11.04 nm), when Zn-containing amalgams were used for restorations, probably leading to a decrease of mechanical properties which might favor crack propagation and deformation. Sound dentin restored with Zn-free amalgams exhibited a substantial increase in length of grain particles (12.44 nm) embedded into dentin crystallites. Zn-containing amalgam placement creates dentin mineralization and the resultant mineral was, utterly, amorphous in nature. Amorphous calcium phosphate will provide a local ion-rich environment which is considered favorable for in situ generation of prenucleation clusters, succeeding further dentin remineralization.