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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/45100

Title: Neuronal apoptosis inhibitory protein (NAIP) localizes to the cytokinetic machinery during cell division
Authors: Abadía Molina, Francisco
Morón Calvente, Virginia
Baird, Stephen D.
Shamim, Fahad
Martín, Francisco
MacKenzie, Alex
Issue Date: 2017
Abstract: The neuronal apoptosis inhibitory protein (NAIP) is a constituent of the inflammasome and a key component of the innate immune system. Here we use immunofluorescence to position NAIP within the cytokinetic apparatus, contiguous to chromosomal passenger complex (CPC), Centralspindlin, PRC1 and KIF4A. During metaphase, NAIP accumulates in the mitotic spindle poles and is shown in spindle microtubules; in anaphase NAIP is detected in the middle of the central spindle. At the end of cytokinesis, NAIP is localized in the outlying region of the stem body, the center of the intercellular bridge formed between daughter cells prior to cellular abscission. We also describe the sustained presence of NAIP mRNA and protein throughout the cell cycle with a significant increase observed in the G2/M phase. Consistent with a role for NAIP in cytokinesis, NAIP overexpression in HeLa cells promotes the acquisition of a multinuclear phenotype. Conversely, NAIP siRNA gene silencing results in an apoptotic lethal phenotype. Our confocal and super resolution stimulated-emission-depletion (STED) examination of mammalian cell cytokinesis demonstrate a potential new role for NAIP in addition to anti-apoptotic and innate immunology functions.
Sponsorship: This work was supported by operating grants from FightSMA, The SMA Foundation, The Canadian Gene Cure Foundation, CIHR and Families of SMA to A.M.
Publisher: Nature Publishing Group
Keywords: Neuronal apoptosis inhibitory protein (NAIP)
Cell proliferation
Cytokinesis
URI: http://hdl.handle.net/10481/45100
ISSN: 2045-2322
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Abadía Molina, F.; et al. Neuronal apoptosis inhibitory protein (NAIP) localizes to the cytokinetic machinery during cell division. Scientific Reports, 7: 39981 (2017). [http://hdl.handle.net/10481/45100]
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