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dc.contributor.advisorNavarro Hilfiker, Sabine Nicolees_ES
dc.contributor.authorGómez Suaga, Patricia Maríaes_ES
dc.contributor.otherUniversidad de Granada. Programa Oficial de Doctorado en: Biomedicina Regenerativaes_ES
dc.contributor.otherConsejo Superior de Investigaciones Científicas (CSIC). Instituto de Parasitología y Biomedicina López-Neyraes_ES
dc.identifier.citationGómez Suaga, P.M. Analysis of LRRK2 Towards understanding the pathogenic mechanisms underlying parkinson's disease: Deregulated autophagy and endocytosis. Granada: Universidad de Granada, 2017. []es_ES
dc.description.abstractAlthough the majority of PD cases are idiopathic, the identification of diseasecausing mutations helps in our understanding of the molecular mechanisms involved in neuronal demise. Mutations in LRRK2 (leucine-rich repeat kinase 2) are found associated with both sporadic and familial Parkinson´s disease (PD). LRRK2 is a multidomain protein characterised by kinase and GTPase activities, with pathogenic mutations localized to both catalytic domains. There is a significant body of evidence correlating altered catalytic activity with cytotoxicity. However, early cellular LRRK2- mediated events which eventually lead to cellular demise remain poorly understood. LRRK2 has been implicated in autophagic and endosomal trafficking pathways in vitro and in vivo, even though the mechanism(s) remain unclear. This thesis describes work towards addressing how pathogenic LRRK2 may cause cellular dysfunction linked to PD pathogenesis, with emphasis on autophagy and endosomal trafficking deficits. First, mutant LRRK2 was found to cause a block in autophagic flux through a pathway involving NAADP (nicotinic acid adenine dinucleotide phosphate)-sensitive channels in acidic late endosomal/lysosomal structures. Pathogenic LRRK2 was found to cause a partial increase in lysosomal pH, and decreased cell survival in the presence of protein aggregation-induced stress. Since autophagy and endocytosis share late endosomes/lysosomes as common end-points, the effect of mutant LRRK2 on endocytosis was evaluated. Pathogenic LRRK2 was found to cause deficits in both early and late events of receptor-mediated endocytosis, including a delay in receptor trafficking out of late endosomes, which become aberrantly elongated. Rab7 is a small GTPase involved in various endosomal trafficking processes, including late endosomal-lysosomal trafficking and retromer-mediated trafficking between late endosomes and the trans-Golgi network. The effects on receptor trafficking could be rescued when overexpressing constitutively active Rab7 or Rab7L1, as well as the Rab7-interacting proteins dynamin2/CIN85, which localize to late endosomes and regulate degradative receptor trafficking. Pathogenic LRRK2 expression was associated with a decrease in Rab7 activity. Alltogether, the present results highlight an important role for pathogenic LRRK2 in deregulating several endolysosomal membrane trafficking events, which may underlie early cellular pathogenesis in PD.es_ES
dc.description.sponsorshipTesis Univ. Granada. Programa Oficial de Doctorado en: Biomedicina Regenerativaes_ES
dc.publisherUniversidad de Granadaes_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Licenseen_US
dc.subjectEnfermedad de Parkinsones_ES
dc.subjectGenética es_ES
dc.subjectEnfermedades lisosomaleses_ES
dc.titleAnalysis of LRRK2 Towards understanding the pathogenic mechanisms underlying parkinson's disease: Deregulated autophagy and endocytosisen_EN
europeana.dataProviderUniversidad de Granada. España.es_ES

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