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Apple polyphenol extract improves insulin sensitivity in vitro and in vivo in animal models of insulin resistance
dc.contributor.author | Manzano, Manuel | es_ES |
dc.contributor.author | Girón González, María Dolores | es_ES |
dc.contributor.author | Vílchez Rienda, José Dámaso | es_ES |
dc.contributor.author | Sevillano Tripero, Natalia | es_ES |
dc.contributor.author | El-Azem de Haro, Nuri | es_ES |
dc.contributor.author | Rueda Cabrera, Ricardo | es_ES |
dc.contributor.author | Salto González, Rafael | es_ES |
dc.contributor.author | López-Pedrosa, José M. | es_ES |
dc.date.accessioned | 2017-02-14T10:20:50Z | |
dc.date.available | 2017-02-14T10:20:50Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Manzano, M.; et al. Apple polyphenol extract improves insulin sensitivity in vitro and in vivo in animal models of insulin resistance. Nutrition and Metabolism, 13: 32 (2016). [http://hdl.handle.net/10481/44824] | es_ES |
dc.identifier.issn | 1743-7075 | |
dc.identifier.uri | http://hdl.handle.net/10481/44824 | |
dc.description.abstract | Background: Apple polyphenols could represent a novel nutritional approach in the management and control of blood glucose, especially in type 2 diabetics. The aim of this study was to test the therapeutic potential of an apple polyphenol extract (APE) in an insulin-resistant rat model and to determine the molecular basis of insulin sensitivity action in skeletal muscle cells. | en_EN |
dc.description.abstract | Methods: Acute effect of APE on the postprandial hyperglycemic response was assayed in 15 week old obese Zucker rats (OZR), by using a meal tolerance test (MTT). The ability of APE to improve whole peripheral insulin sensitivity was also assayed in a chronic study by using the euglycemic-hyperinsulinemic clamp technique. To elucidate the molecular mechanisms, rat L6 myotubes were used. Glucose uptake was measured by using 2-[3H]-Deoxy-Glucose (2-DG) and specific inhibitors, as well as phosphorylation status of key kinases, were used to determine the implicated signaling pathway. | en_EN |
dc.description.abstract | Results: In vivo study showed that nutritional intervention with APE induced an increase of insulin sensitivity with an increase of glucose infusion rate (GIR) of 45 %. Additionally, in vitro results showed a synergistic effect between APE and insulin as well as increased glucose uptake through GLUT4 translocation in muscle cells. This translocation was mediated by phosphatydil inositol 3-kinase (PI3K) and peroxisome proliferator-activated receptor-gamma (PPARγ) signaling pathways. | en_EN |
dc.description.abstract | Conclusions: As a whole, this study describes the mechanisms involved in the insulin sensitizing effect of APE, which could be considered a promising ingredient for inclusion in nutritional products focused on the management of chronic diseases such as diabetes. | en |
dc.description.sponsorship | This research was supported by funds from Abbott Laboratories S.A. | en_EN |
dc.language.iso | eng | es_ES |
dc.publisher | Biomed Central | es_ES |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License | en_EN |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | en_EN |
dc.subject | Apple polyphenol extract | en_EN |
dc.subject | Insulin sensitivity | en_EN |
dc.subject | Blood glucose uptake | en_EN |
dc.subject | Skeletal muscle | en_EN |
dc.subject | Diabetes | en_EN |
dc.title | Apple polyphenol extract improves insulin sensitivity in vitro and in vivo in animal models of insulin resistance | en_EN |
dc.type | info:eu-repo/semantics/article | en_EN |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en_EN |
dc.identifier.doi | 10.1186/s12986-016-0088-8 |