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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/44799

Title: Selection of the N-Acylhomoserine Lactone-Degrading Bacterium Alteromonas stellipolaris PQQ-42 and of Its Potential for Biocontrol in Aquaculture
Authors: Torres, Marta
Rubio-Portillo, Esther
Antón, Josefa
Ramos-Esplá, Alfonso A.
Quesada Arroquia, Emilia
Llamas Company, Inmaculada
Issue Date: 2016
Abstract: The production of virulence factors by many pathogenic microorganisms depends on the intercellular communication system called quorum sensing, which involves the production and release of signal molecules known as autoinducers. Based on this, new-therapeutic strategies have emerged for the treatment of a variety of infections, such as the enzymatic degradation of signaling molecules, known as quorum quenching (QQ). In this study, we present the screening of QQ activity amongst 450 strains isolated from a bivalve hatchery in Granada (Spain), and the selection of the strain PQQ-42, which degrades a wide range of N-acylhomoserine lactones (AHLs). The selected strain, identified as Alteromonas stellipolaris, degraded the accumulation of AHLs and reduced the production of protease and chitinase and swimming motility of a Vibrio species in co-cultivation experiments in vitro. In the bio-control experiment, strain PQQ-42 significantly reduced the pathogenicity of Vibrio mediterranei VibC-Oc-097 upon the coral Oculina patagonica showing a lower degree of tissue damage (29.25 ± 14.63%) in its presence, compared to when the coral was infected with V. mediterranei VibC-Oc-097 alone (77.53 ± 13.22%). Our results suggest that this AHL-degrading bacterium may have biotechnological applications in aquaculture.
Sponsorship: This research was supported by grants from the Dirección General de Investigación Científica y Técnica (BIO2011-12879E; AGL2015-68806-R) and from the Plan Andaluz de Investigación (P07-CVI-03150; CVI06226), Spain. MT is supported by a FPU fellowship from the Spanish Ministry of Science and Education (FPU13-0466).
Publisher: Frontiers Media
Description: The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fmicb.2016.00646
Keywords: Quorum quenching
Aquaculture
Vibrio
Biocontrol
N-acylhomoserine lactone
URI: http://hdl.handle.net/10481/44799
ISSN: 1664-302X
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Torres, M.; et al. Selection of the N-Acylhomoserine Lactone-Degrading Bacterium Alteromonas stellipolaris PQQ-42 and of Its Potential for Biocontrol in Aquaculture. Frontiers in Microbiology, 7: 646 (2016). [http://hdl.handle.net/10481/44799]
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