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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/44771

Title: Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion
Authors: Chamorro, Virginia
Pandolfi, Rachele
Moreno, Laura
Barreira, Bianca
Martínez-Ramas, Andrea
Morales-Cano, Daniel
Ruiz-Cabello, Jesús
Lorente, José Ángel
Duarte, Juan
Cogolludo, Ángel
Álvarez-Sala, José Luis
Pérez-Vizcaíno, Francisco
Issue Date: 2016
Abstract: Background: We hypothesized that treatment with quercetin could result in improved hemodynamics, lung inflammatory parameters and mortality in a rat model of hemorrhagic shock. Methods: Rats were anesthetized (80 mg/kg ketamine plus 8 mg/kg xylazine i.p.). The protocol included laparotomy for 15 min (trauma), hemorrhagic shock (blood withdrawal to reduce the mean arterial pressure to 35 mmHg) for 75 min and resuscitation by re-infusion of all the shed blood plus lactate Ringer for 90 min. Intravenous quercetin (50 mg/kg) or vehicle were administered during resuscitation. Results: There was a trend for increased survival 84.6% (11/13) in the treated group vs. the shock group 68.4% (13/19, p > 0.05 Kaplan–Meier). Quercetin fully prevented the development of lung edema. The activity of aSMase was increased in the shock group compared to the sham group and the quercetin prevented this effect. However, other inflammatory markers such as myeloperoxidase activity, interleukin-6 in plasma or bronchoalveolar fluid were similar in the sham and shock groups. We found no bacterial DNA in plasma in these animals. Conclusions: Quercetin partially prevented the changes in blood pressure and lung injury in shock associated to hemorrhage and reperfusion.
Sponsorship: Supported by FundaciónMutuaMadrileña (AP102962012), SpanishMINECO(SAF 2011-28150; SAF2014-55399R; SAF2014-58920) and ISCIII (CP12/03304, FIS 15/1492).
Keywords: Systemic arterial pressure
Lung edema
Hemorrhage
Trauma
Reperfusion
URI: http://hdl.handle.net/10481/44771
ISSN: 1420-3049
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Chamorro, V.; et al. Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion. Molecules, 21(12): 1739 (2016). [http://hdl.handle.net/10481/44771]
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