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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/40069

Title: Estudio bioenergético mitocondrial en la sepsis experimental y efecto protector de la melatonina
Authors: Doerrier Velasco, Carolina Annaliese
Direction: Acuña Castroviejo, Darío
Escames Rosa, Germaine
Collaborator: Universidad de Granada. Programa Oficial de Doctorado en: Biomedicina
Issue Date: 2016
Submitted Date: 6-Mar-2015
Abstract: The present work was focused in the study of mitochondrial function during sepsis. Moreover, was evaluated the properties of melatonin against mitochondrial failure during sepsis. The objectives of this research were: 1. To determine the mitochondrial bioenergetics in liver and heart of mice at early and late phases of sepsis. 2. To identify mitochondrial substrate-dependent behavior during septic process in these tissues. 3. To assess the protective effect of melatonin administration to mice on mitochondrial function of liver and heart of septic mice. 4. To evaluate the mechanistics effects of melatonin on mitochondria using different substrate combinations in liver and heart of septic mice.
The present thesis reports on the study of mitochondrial function (of 3 mo. C57BL/6 mice) in liver and heart mouse at 8 and 24 h of sepsis induction by cecal ligation and puncture (CLP). Mainly, mitochondrial function was evaluated assessing mitochondrial respiration by high resolution respirometry (HRR). Other bioenergetic parameters including cytochrome a + a3, b, and c + c1 content, mitochondrial mass, and mitochondrial supercomplexes (SC) formation, were analyzed. Regarding to an effective treatment, we assess melatonin, a recognize molecule with important pleiotropic functions.
Sponsorship: Tesis Univ. Granada. Programa Oficial de Doctorado en: Biomedicina
Durante la realización del siguiente trabajo, la Lda. Carolina Anneliese Doerrier Velasco fue financiada por las siguientes fuentes: RETICEF (2009-2011); Beca del programa de doctorado en Biomedicina, Contrato de técnico de apoyo investigación FIBAO (2011-2013); Proyecto P07-CTS-03135 de la Junta de Andalucíal, Consejería de Innovación, Ciencia y Empresa; Proyecto PI08-1664 de ISCIII.
Publisher: Universidad de Granada
Keywords: Melatonina
Biomedicina
Mitocondrias
Estrés oxidativo
Sepsis
UDC: 616
542
3207.07
URI: http://hdl.handle.net/10481/40069
ISBN: 9788491250814
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Doerrier Velasco, C.A. Estudio bioenergético mitocondrial en la sepsis experimental y efecto protector de la melatonina. Granada: Universidad de Granada, 2016. [http://hdl.handle.net/10481/40069]
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