Universidad de Granada Digibug
 

Repositorio Institucional de la Universidad de Granada >
1.-Investigación >
Departamentos, Grupos de Investigación e Institutos >
Departamento de Ingeniería Civil >
DIC - Artículos >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/38031

Title: 454-Pyrosequencing Analysis of Bacterial Communities from Autotrophic Nitrogen Removal Bioreactors Utilizing Universal Primers: Effect of Annealing Temperature
Authors: González-Martínez, Alejandro
Rodríguez-Sánchez, Alejandro
Rodelas González, María Belén
Abbas, Ben A.
Martínez-Toledo, María Victoria
Loosdrecht, Mark C. M. van
Osorio, Francisco
González López, Jesús
Issue Date: 2015
Abstract: Identification of anaerobic ammonium oxidizing (anammox) bacteria by molecular tools aimed at the evaluation of bacterial diversity in autotrophic nitrogen removal systems is limited by the difficulty to design universal primers for the Bacteria domain able to amplify the anammox 16S rRNA genes. A metagenomic analysis (pyrosequencing) of total bacterial diversity including anammox population in five autotrophic nitrogen removal technologies, two bench-scale models (MBR and Low Temperature CANON) and three full-scale bioreactors (anammox, CANON, and DEMON), was successfully carried out by optimization of primer selection and PCR conditions (annealing temperature). The universal primer 530F was identified as the best candidate for total bacteria and anammox bacteria diversity coverage. Salt-adjusted optimum annealing temperature of primer 530F was calculated (47°C) and hence a range of annealing temperatures of 44–49°C was tested. Pyrosequencing data showed that annealing temperature of 45°C yielded the best results in terms of species richness and diversity for all bioreactors analyzed.
Publisher: Hindawi Publishing Corporation
Keywords: Anaerobic ammonium oxidizing (Anammox)
Molecular tools
Temperature
URI: http://hdl.handle.net/10481/38031
ISSN: 2314-6133
2314-6141
Citation: González-Martínez, A.; et al. 454-Pyrosequencing Analysis of Bacterial Communities from Autotrophic Nitrogen Removal Bioreactors Utilizing Universal Primers: Effect of Annealing Temperature. BioMed Research International, 2015: 892013 (2015). [http://hdl.handle.net/10481/38031]
Appears in Collections:DIC - Artículos

Files in This Item:

File Description SizeFormat
GonzalezMartinez_Anammox.pdf1.28 MBAdobe PDFView/Open
Recommend this item

This item is licensed under a Creative Commons License
Creative Commons

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! OpenAire compliant DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard - Feedback

© Universidad de Granada