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dc.contributor.authorPeralta Pereira, Laura María
dc.contributor.authorMourier, Eve
dc.contributor.authorRichard, Chrisophe
dc.contributor.authorCharpigny, Gilles
dc.contributor.authorLarcher, Thibaut
dc.contributor.authorAït-Belkacem, Dora
dc.contributor.authorBalla, Naveen K.
dc.contributor.authorBrasselet, Sophie
dc.contributor.authorTanter, Mickael
dc.contributor.authorMuller, Marie
dc.contributor.authorChavatte-Palmer, Pascale
dc.date.accessioned2015-09-28T10:02:30Z
dc.date.available2015-09-28T10:02:30Z
dc.date.issued2015
dc.identifier.citationPeralta Pereira, L.; et al. In Vivo Evaluation of Cervical Stiffness Evolution during Induced Ripening Using Shear Wave Elastography, Histology and 2 Photon Excitation Microscopy: Insight from an Animal Model. Plos One, 10(8): e0133377 (2015). [http://hdl.handle.net/10481/37681]es_ES
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10481/37681
dc.description.abstractPrematurity affects 11% of the births and is the main cause of infant mortality. On the opposite case, the failure of induction of parturition in the case of delayed spontaneous birth is associated with fetal suffering. Both conditions are associated with precocious and/or delayed cervical ripening. Quantitative and objective information about the temporal evolution of the cervical ripening may provide a complementary method to identify cases at risk of preterm delivery and to assess the likelihood of successful induction of labour. In this study, the cervical stiffness was measured in vivo in pregnant sheep by using Shear Wave Elastography (SWE). This technique assesses the stiffness of tissue through the measurement of shear waves speed (SWS). In the present study, 9 pregnant ewes were used. Cervical ripening was induced at 127 days of pregnancy (term: 145 days) by dexamethasone injection in 5 animals, while 4 animals were used as control. Elastographic images of the cervix were obtained by two independent operators every 4 hours during 24 hours after injection to monitor the cervical maturation induced by the dexamethasone. Based on the measurements of SWS during vaginal ultrasound examination, the stiffness in the second ring of the cervix was quantified over a circular region of interest of 5 mm diameter. SWS was found to decrease significantly in the first 4–8 hours after dexamethasone compared to controls, which was associated with cervical ripening induced by dexamethasone (from 1.779 m/s ± 0.548 m/s, p < 0.0005, to 1.291 m/s ± 0.516 m/s, p < 0.000). Consequently a drop in the cervical elasticity was quantified too (from 9.5 kPa ± 0.9 kPa, p < 0.0005, to 5.0 kPa ± 0.8 kPa, p < 0.000). Moreover, SWE measurements were highly reproducible between both operators at all times. Cervical ripening induced by dexamethasone was confirmed by the significant increase in maternal plasma Prostaglandin E2 (PGE2), as evidenced by the assay of its metabolite PGEM. Histological analyses and two-photon excitation microscopy, combining both Second Harmonic Generation (SHG) and Two-photon Fluorescence microscopy (2PF) contrasts, were used to investigate, at the microscopic scale, the structure of cervical tissue. Results show that both collagen and 2PF-active fibrillar structures could be closely related to the mechanical properties of cervical tissue that are perceptible in elastography. In conclusion, SWE may be a valuable method to objectively quantify the cervical stiffness and as a complementary diagnostic tool for preterm birth and for labour induction success.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS)es_ES
dc.subjectStiffnesses_ES
dc.subjectCervixes_ES
dc.subjectCollagenses_ES
dc.subjectChoriones_ES
dc.subjectHistology es_ES
dc.subjectPreterm birthes_ES
dc.subjectProstaglandines_ES
dc.subjectUltrasound imaginges_ES
dc.titleIn Vivo Evaluation of Cervical Stiffness Evolution during Induced Ripening Using Shear Wave Elastography, Histology and 2 Photon Excitation Microscopy: Insight from an Animal Modeles_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.1371/journal.pone.0133377


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