Universidad de Granada Digibug
 

Repositorio Institucional de la Universidad de Granada >
1.-Investigación >
Departamentos, Grupos de Investigación e Institutos >
Departamento de Ciencias de la Computación e Inteligencia Artificial >
DCCIA - Artículos >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/37290

Title: Jarid2 Coordinates Nanog Expression and PCP/Wnt Signaling Required for Efficient ESC Differentiation and Early Embryo Development
Authors: Landeira, David
Bagci, Hakan
Malinowski, Andrzej R.
Brown, Karen E.
Soza-Ried, Jorge
Feytout, Amelie
Webster, Zoe
Ndjetehe, Elodie
Cantone, Irene
Asenjo, Helena G.
Brockdorff, Neil
Carroll, Thomas
Merkenschlager, Matthias
Fisher, Amanda G.
Issue Date: 2015
Abstract: Jarid2 is part of the Polycomb Repressor complex 2 (PRC2) responsible for genome-wide H3K27me3 deposition. Unlike other PRC2-deficient embryonic stem cells (ESCs), however, Jarid2-deficient ESCs show a severe differentiation block, altered colony morphology, and distinctive patterns of deregulated gene expression. Here, we show that Jarid2−/− ESCs express constitutively high levels of Nanog but reduced PCP signaling components Wnt9a, Prickle1, and Fzd2 and lowered β-catenin activity. Depletion of Wnt9a/Prickle1/Fzd2 from wild-type ESCs or overexpression of Nanog largely phenocopies these cellular defects. Co-culture of Jarid2−/− with wild-type ESCs restores variable Nanog expression and β-catenin activity and can partially rescue the differentiation block of mutant cells. In addition, we show that ESCs lacking Jarid2 or Wnt9a/Prickle1/Fzd2 or overexpressing Nanog induce multiple ICM formation when injected into normal E3.5 blastocysts. These data describe a previously unrecognized role for Jarid2 in regulating a core pluripotency and Wnt/PCP signaling circuit that is important for ESC differentiation and for pre-implantation development.
Sponsorship: This study was supported by the Medical Research Council, the ERC (H.B. and J.S.-R.) (#294627), the HFSP (I.C.), and the Spanish Ministry of Economy and Competitiveness (D.L. and H.G.A.) (RYC-2012-10019; SAF2013-40891-R).
Publisher: Elsevier
Keywords: Cell self-renewal
Epiblast stem-cells
Beta-catenin
Gene expression
Polycom repression
Pluripotency
URI: http://hdl.handle.net/10481/37290
ISSN: 2211-1247
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Landeira, D.; et al. Jarid2 Coordinates Nanog Expression and PCP/Wnt Signaling Required for Efficient ESC Differentiation and Early Embryo Development. Cell Reports, 12(4): 573-586 (2015). [http://hdl.handle.net/10481/37290]
Appears in Collections:DCCIA - Artículos

Files in This Item:

File Description SizeFormat
Landeira_EmbryoDevelopment.pdf3.35 MBAdobe PDFView/Open
Recommend this item

This item is licensed under a Creative Commons License
Creative Commons

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! OpenAire compliant DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard - Feedback

© Universidad de Granada