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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/37271

Title: Microglial Activation Promotes Cell Survival in Organotypic Cultures of Postnatal Mouse Retinal Explants
Authors: Ferrer-Martín, Rosa María
Martín-Oliva, David
Sierra, Ana
Carrasco Sierra, María del Carmen
Martín-Estebané, María
Calvente Iglesias, Ruth
Martín-Guerrero, Sandra M.
Marín-Teva, José Luis
Navascués Martínez, Julio
Cuadros Ojeda, Miguel Ángel
Issue Date: 2015
Abstract: The role of microglia during neurodegeneration remains controversial. We investigated whether microglial cells have a neurotoxic or neuroprotective function in the retina. Retinal explants from 10-day-old mice were treated in vitro with minocycline to inhibit microglial activation, with LPS to increase microglial activation, or with liposomes loaded with clodronate (Lip-Clo) to deplete microglial cells. Flow cytometry was used to assess the viability of retinal cells in the explants and the TUNEL method to show the distribution of dead cells. The immunophenotypic and morphological features of microglia and their distribution were analyzed with flow cytometry and immunocytochemistry. Treatment of retinal explants with minocycline reduced microglial activation and simultaneously significantly decreased cell viability and increased the presence of TUNEL-labeled cell profiles. This treatment also prevented the migration of microglial cells towards the outer nuclear layer, where cell death was most abundant. The LPS treatment increased microglial activation but had no effect on cell viability or microglial distribution. Finally, partial microglial removal with Lip-Clo diminished the cell viability in the retinal explants, showing a similar effect to that of minocycline. Hence, cell viability is diminished in retinal explants cultured in vitro when microglial cells are removed or their activation is inhibited, indicating a neurotrophic role for microglia in this system.
Sponsorship: This work was supported by Junta de Andalucía, Spain, Grant P07-CVI-03008 (http://www.juntadeandalucia.es/organismo​s/economiainnovacioncienciayempleo.html), and Ministerio de Economía y Competitividad, Spain, Grant BFU2010-19981 (http://www.mineco.gob.es/portal/site/min​eco/idi).
Publisher: Public Library of Science (PLOS)
Keywords: Microglial cells
Retina
Flow cytometry
Phtoreceptors
Cell death
Macrophages
Retinal degeneration
Liposomes
URI: http://hdl.handle.net/10481/37271
ISSN: 1932-6203
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Ferrer-Martín, R.M.; et al. Microglial Activation Promotes Cell Survival in Organotypic Cultures of Postnatal Mouse Retinal Explants. Plos One, 10(8): e0135238 (2015). [http://hdl.handle.net/10481/37271]
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