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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/37239

Title: Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions
Authors: Mokhtari, Khalida
Rufino Palomares, Eva Encarnación
Pérez-Jiménez, Amalia
Reyes Zurita, Fernando Jesús
Figuera, Celeny
García-Salguero, Leticia
Medina, Pedro P.
Peragón Sánchez, Juan
Lupiáñez Cara, José Antonio
Issue Date: 2015
Abstract: Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA.The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense.However, at higher dosagesMAinduced cellular damage by apoptosis as seen in the results obtained.
Sponsorship: This study has been supported, in part, by funds of the consolidated Research Group BIO-157, from the General Secretariat of Universities, Research and Technology of the Ministry of Economy, Innovation, Science and Employment Government of the Junta de Andaluc´ıa (Spain), and by the Research Contract no. C-3650-00 under the program FEDER-INNTERCONECTA from the Spanish Government and European Union FEDER funds. Amalia P´erez-Jim´enez is a recipient of a postdoctoral research fellowship Torres- Quevedo no. PTQ 12-05739.
Publisher: Hindawi Publishing Corporation
Keywords: Maslinic acid (MA)
Melanoma cancer
Reactive oxygen species (ROS)
URI: http://hdl.handle.net/10481/37239
ISSN: 1741-427X
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Mokhtari, K.; et al. Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions. Evidence-Based Complementary and Alternative Medicine, 2015: 272457 (2015). [http://hdl.handle.net/10481/37239]
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