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dc.contributor.authorLi, Zhiguang
dc.contributor.authorQin, Taichun
dc.contributor.authorWang, Kejian
dc.contributor.authorHackenberg , Michael 
dc.contributor.authorYan, Jian
dc.contributor.authorGao, Yuan
dc.contributor.authorYu, Li-Rong
dc.contributor.authorSi, Zhengian Su
dc.contributor.authorChen, Tao
dc.date.accessioned2015-06-25T12:34:48Z
dc.date.available2015-06-25T12:34:48Z
dc.date.issued2015
dc.identifier.citationLi, Z.; et al. Integrated microRNA, mRNA, and protein expression profiling reveals microRNA regulatory networks in rat kidney treated with a carcinogenic dose of aristolochic acid. BMC Genomics, 16: 365 (2015). []es_ES
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/10481/36753
dc.description.abstractBackground: Aristolochic Acid (AA), a natural component of Aristolochia plants that is found in a variety of herbal remedies and health supplements, is classified as a Group 1 carcinogen by the International Agency for Research on Cancer. Given that microRNAs (miRNAs) are involved in cancer initiation and progression and their role remains unknown in AA-induced carcinogenesis, we examined genome-wide AA-induced dysregulation of miRNAs as well as the regulation of miRNAs on their target gene expression in rat kidney.es_ES
dc.description.abstractResults: We treated rats with 10 mg/kg AA and vehicle control for 12 weeks and eight kidney samples (4 for the treatment and 4 for the control) were used for examining miRNA and mRNA expression by deep sequencing, and protein expression by proteomics. AA treatment resulted in significant differential expression of miRNAs, mRNAs and proteins as measured by both principal component analysis (PCA) and hierarchical clustering analysis (HCA). Specially, 63 miRNAs (adjusted p value < 0.05 and fold change > 1.5), 6,794 mRNAs (adjusted p value < 0.05 and fold change > 2.0), and 800 proteins (fold change > 2.0) were significantly altered by AA treatment. The expression of 6 selected miRNAs was validated by quantitative real-time PCR analysis. Ingenuity Pathways Analysis (IPA) showed that cancer is the top network and disease associated with those dysregulated miRNAs. To further investigate the influence of miRNAs on kidney mRNA and protein expression, we combined proteomic and transcriptomic data in conjunction with miRNA target selection as confirmed and reported in miRTarBase. In addition to translational repression and transcriptional destabilization, we also found that miRNAs and their target genes were expressed in the same direction at levels of transcription (169) or translation (227). Furthermore, we identified that up-regulation of 13 oncogenic miRNAs was associated with translational activation of 45 out of 54 cancer-related targets.es_ES
dc.description.abstractConclusions: Our findings suggest that dysregulated miRNA expression plays an important role in AA-induced carcinogenesis in rat kidney, and that the integrated approach of multiple profiling provides a new insight into a post-transcriptional regulation of miRNAs on their target repression and activation in a genome-wide scale.es_ES
dc.language.isoenges_ES
dc.publisherBiomed Centrales_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Licensees_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es_ES
dc.subjectAristolochic acides_ES
dc.subjectCarcinogenesis es_ES
dc.subjectKidney tumores_ES
dc.subjectmicroRNAes_ES
dc.subjectProteomicses_ES
dc.subjectDeep-Sequencinges_ES
dc.subjectRNA arrayes_ES
dc.subjectTarget predictiones_ES
dc.subjectRates_ES
dc.titleIntegrated microRNA, mRNA, and protein expression profiling reveals microRNA regulatory networks in rat kidney treated with a carcinogenic dose of aristolochic acides_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.1186/s12864-015-1516-2


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