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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/36690

Title: Number of Nanoparticles per Cell through a Spectrophotometric Method - A key parameter to Assess Nanoparticle-based Cellular Assays
Authors: Unciti-Broceta, Juan Diego
Cano-Cortés, Victoria
Altea-Manzano, Patricia
Pernagallo, Salvatore
Díaz-Mochón, Juan J.
Sánchez-Martín, Rosario M.
Issue Date: 2015
Abstract: Engineered nanoparticles (eNPs) for biological and biomedical applications are produced from functionalised nanoparticles (NPs) after undergoing multiple handling steps, giving rise to an inevitable loss of NPs. Herein we present a practical method to quantify nanoparticles (NPs) number per volume in an aqueous suspension using standard spectrophotometers and minute amounts of the suspensions (up to 1 μL). This method allows, for the first time, to analyse cellular uptake by reporting NPs number added per cell, as opposed to current methods which are related to solid content (w/V) of NPs. In analogy to the parameter used in viral infective assays (multiplicity of infection), we propose to name this novel parameter as multiplicity of nanofection.
Sponsorship: JJDM thanks Spanish Ministerio de Economía y Competitividad for a Ramon y Cajal Fellowship and for supporting this work partially by Grant CTQ2012-34778. This research was partially supported by Marie Curie Career Integration Grants within the 7th European Community Framework Programme (FP7-PEOPLE-2011-CIG-Project Number 294142 and FP7-PEOPLE-2012-CIG-Project Number 322276) to RMSM and JJDM, respectively. This research was partially supported by the Consejería de Economía, Innovación y Ciencia de la Junta de Andalucía (BIO-1778) to JJDM. RMSM and JDUB thank CEI Biotic Granada for funding P_BS_54 and mP_BS_37 projects. JDUB thanks Spanish Ministerio de Economía y Competitividad for a Torres Quevedo fellowship (PTQ-13-06046).
Publisher: Nature Publishing Group
Keywords: Nanoparticles
Biomarker research
URI: http://hdl.handle.net/10481/36690
ISSN: 2045-2322
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Unciti-Broceta, J.D.; et al. Number of Nanoparticles per Cell through a Spectrophotometric Method - A key parameter to Assess Nanoparticle-based Cellular Assays. Scientific Reports, 5: 10091 (2015). [http://hdl.handle.net/10481/36690]
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