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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/36564

Title: Genome-Wide Expression in Visceral Adipose Tissue from Obese Prepubertal Children
Authors: Aguilera García, Concepción
Gómez Llorente, Carolina
Tofe, Inés
Gil-Campos, Mercedes
Cañete, Ramón
Gil Hernández, Ángel
Issue Date: 2015
Abstract: Characterization of the genes expressed in adipose tissue (AT) is key to understanding the pathogenesis of obesity and to developing treatments for this condition. Our objective was to compare the gene expression in visceral AT (VAT) between obese and normal-weight prepubertal children. A total of fifteen obese and sixteen normal-weight children undergoing abdominal elective surgery were selected. RNA was extracted from VAT biopsies. Microarray experiments were independently performed for each sample (six obese and five normal-weight samples). Validation by quantitative PCR (qPCR) was performed on an additional 10 obese and 10 normal-weight VAT samples. Of 1276 differentially expressed genes (p < 0.05), 245 were more than two-fold higher in obese children than in normal-weight children. As validated by qPCR, expression was upregulated in genes involved in lipid and amino acid metabolism (CES1, NPRR3 and BHMT2), oxidative stress and extracellular matrix regulation (TNMD and NQO1), adipogenesis (CRYAB and AFF1) and inflammation (ANXA1); by contrast, only CALCRL gene expression was confirmed to be downregulated. In conclusion, this study in prepubertal children demonstrates the up- and down-regulation of genes that encode molecules that were previously proposed to influence the pathogenesis of adulthood obesity, as well as previously unreported dysregulated genes that may be candidate genes in the aetiology of obesity.
Sponsorship: This work was supported by Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I + D + I), Instituto de Salud Carlos III-Fondo de Investigación Sanitaria (FONDOS FEDER) Projects no PI 020826 and PI051968 and Redes temáticas de investigación cooperativa RETIC (Red SAMID RD12/0026/0015). CG-L is a recipient of a fellowship from Plan Propio UGR.
Publisher: MDPI
Keywords: Obesity
Visceral adipose tissue
Prepubertal children
Microarray
URI: http://hdl.handle.net/10481/36564
ISSN: 1422-0067
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Aguilera, C.M.; et al. Genome-Wide Expression in Visceral Adipose Tissue from Obese Prepubertal Children. International Journal of Molecular Sciences, 16(4): 7723-7737 (2015). [http://hdl.handle.net/10481/36564]
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