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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/35876

Title: Determination of Polar Compounds in Guava Leaves Infusions and Ultrasound Aqueous Extract by HPLC-ESI-MS
Authors: Díaz-de-Cerio, Elixabet
Verardo, Vito
Gómez-Caravaca, Ana María
Fernández Gutiérrez, Alberto
Segura Carretero, Antonio
Issue Date: 2015
Abstract: Literature lacks publications about polar compounds content in infusion or guava leaves tea. Because of that, a comparison between different times of infusion and a conventional ultrasound aqueous extract was carried out. Several polar compounds have been identified by HPLC-ESI-MS and their antioxidant activity was evaluated by FRAP and ABTS assays. Four different classes of phenolic compounds (gallic and ellagic acid derivatives, flavonols, flavanones, and flavan-3-ols) and some benzophenones were determined. The quantification results reported that the order, in terms of concentration of the classes of polar compounds in all samples, was flavonols > flavan-3-ols > gallic and ellagic acid derivatives > benzophenones > flavanones. As expected, the aqueous extract obtained by sonication showed the highest content in the compounds studied. Significative differences were noticed about the different times of infusion and five minutes was the optimal time to obtain the highest content in polar compounds using this culinary method. All the identified compounds, except HHDP isomers and naringenin, were positively correlated with antioxidant activity.
Sponsorship: This work was funded by the Project cofinanced by FEDER-Andalucía 2007–2013 (Cod. 461100) and Andalusian Regional Government Council of Innovation and Science (P11-CTS-7625). The author Elixabet Díaz-de-Cerio also would like to thank the CEIBiotic for the “Ayudas a la Enseñanza Práctica” Grant (CADP2-71). Ana María Gómez-Caravaca and Vito Verardo thank the Spanish Ministry of Economy and Competitiveness (MINECO) for “Juan de la Cierva” postdoctoral contract.
Publisher: Hindawi Publishing Corporation
Keywords: Polar compounds
Antioxidant activity
URI: http://hdl.handle.net/10481/35876
ISBN: 2090-9063
ISSN: 2090-9071
Citation: Díaz de Cerio, E.; et al. Determination of Polar Compounds in Guava Leaves Infusions and Ultrasound Aqueous Extract by HPLC-ESI-MS. Journal of Chemistry, 2015: 250919 (2015). [http://hdl.handle.net/10481/35876]
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