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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/35340

Title: Identification of Histological Patterns in Clinically Affected and Unaffected Palm Regions in Dupuytren's Disease
Authors: Alfonso-Rodríguez, Camilo Andrés
Garzón Bello, Ingrid Johanna
Garrido-Gómez, Juan
Ximenes Oliveira, Ana Celeste
Martín-Piedra, Miguel Ángel
Scionti, Giuseppe
Carriel Araya, Víctor
Hernández-Cortés, Pedro
Campos Muñoz, Antonio
Alaminos Mingorance, Miguel
Issue Date: 2014
Abstract: Dupuytren's disease is a fibro-proliferative disease characterized by a disorder of the extracellular matrix (ECM) and high myofibroblast proliferation. However, studies failed to determine if the whole palm fascia is affected by the disease. The objective of this study was to analyze several components of the extracellular matrix of three types of tissues—Dupuytren's diseased contracture cords (DDC), palmar fascia clinically unaffected by Dupuytren's disease contracture (NPF), and normal forehand fascia (NFF). Histological analysis, quantification of cells recultured from each type of tissue, mRNA microarrays and immunohistochemistry for smooth muscle actin (SMA), fibrillar ECM components and non-fibrillar ECM components were carried out. The results showed that DDC samples had abundant fibrosis with reticular fibers and few elastic fibers, high cell proliferation and myofibroblasts, laminin and glycoproteins, whereas NFF did not show any of these findings. Interestingly, NPF tissues had more cells showing myofibroblasts differentiation and more collagen and reticular fibers, laminin and glycoproteins than NFF, although at lower level than DDC, with similar elastic fibers than DDC. Immunohistochemical expression of decorin was high in DDC, whereas versican was highly expressed NFF, with no differences for aggrecan. Cluster analysis revealed that the global expression profile of NPF was very similar to DDC, and reculturing methods showed that cells corresponding to DDC tissues proliferated more actively than NPF, and NPF more actively than NFF. All these results suggest that NPF tissues may be affected, and that a modification of the therapeutic approach used for the treatment of Dupuytren's disease should be considered.
Sponsorship: This work was supported by CTS-115 (Tissue Engineering Group), University of Granada/Spain.
Publisher: Public Library of Science (PLOS)
Keywords: Collagens
Sulfates
Proetoglycans
Gene expression
Fibroblasts
Glycoproteins
Immunohistochemical analysis
Staining
URI: http://hdl.handle.net/10481/35340
ISSN: 1932-6203
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Alfonso-Rodríguez, C.A.; et al. Identification of Histological Patterns in Clinically Affected and Unaffected Palm Regions in Dupuytren's Disease. Plos One, 9(11): e112457 (2014). [http://hdl.handle.net/10481/35340]
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