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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/35300

Title: Bacterial Nucleoid-Associated Protein Uncouples Transcription Levels from Transcription Timing
Authors: Zwir, Igor
Yeo, Won-Sik
Shin, Dongwoo
Latifi, Tammy
Huang, Henry
Groisman, Eduardo A.
Issue Date: 2014
Abstract: The histone-like nucleoid-structuring (H-NS) protein binds to horizontally acquired genes in the bacterium Salmonella enterica serovar Typhimurium, silencing their expression. We now report that overcoming the silencing effects of H-NS imposes a delay in the expression of genes activated by the transcriptional regulator PhoP. We determine that PhoP-activated genes ancestral to Salmonella are expressed before those acquired horizontally. This expression timing reflects the in vivo occupancy of the corresponding promoters by the PhoP protein. These results are surprising because some of these horizontally acquired genes reached higher mRNA levels than ancestral genes expressed earlier and were transcribed from promoters harboring PhoP-binding sites with higher in vitro affinity for the PhoP protein. Our findings challenge the often-made assumption that for genes coregulated by a given transcription factor, early genes are transcribed to higher mRNA levels than those transcribed at later times. Moreover, they provide a singular example of how gene ancestry can impact expression timing.
Sponsorship: This work was supported, in part, by grant AI49561 from the National Institutes of Health to E.A.G., who is an Investigator of the Howard Hughes Medical Institute.
Publisher: American Academy of Microbiology
Keywords: Salmonella
Protein domains
Gene expression
URI: http://hdl.handle.net/10481/35300
ISSN: 2150-7511
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Zwir, I.; et al. Bacterial Nucleoid-Associated Protein Uncouples Transcription Levels from Transcription Timing. mBio, 5(5): e01485 (2014). [http://hdl.handle.net/10481/35300]
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