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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/34958

Title: Expression Profiling of Rectal Tumors Defines Response to Neoadjuvant Treatment Related Genes
Authors: Palma Carazo, Pablo
Cano Gutiérrez, Carlos
Conde-Muíño, Raquel
Comino, Ana
Bueno Laraño, Pablo
Ferrón Orihuela, José Antonio
Cuadros Celorrio, Marta
Issue Date: 2014
Abstract: To date, no effective method exists that predicts the response to preoperative chemoradiation (CRT) in locally advanced rectal cancer (LARC). Nevertheless, identification of patients who have a higher likelihood of responding to preoperative CRT could be crucial in decreasing treatment morbidity and avoiding expensive and time-consuming treatments. The aim of this study was to identify signatures or molecular markers related to response to pre-operative CRT in LARC. We analyzed the gene expression profiles of 26 pre-treatment biopsies of LARC (10 responders and 16 non-responders) without metastasis using Human WG CodeLink microarray platform. Two hundred and fifty seven genes were differentially over-expressed in the responder patient subgroup. Ingenuity Pathway Analysis revealed a significant ratio of differentially expressed genes related to cancer, cellular growth and proliferation pathways, and c-Myc network. We demonstrated that high Gng4, c-Myc, Pola1, and Rrm1 mRNA expression levels was a significant prognostic factor for response to treatment in LARC patients (p<0.05). Using this gene set, we were able to establish a new model for predicting the response to CRT in rectal cancer with a sensitivity of 60% and 100% specificity. Our results reflect the value of gene expression profiling to gain insight about the molecular pathways involved in the response to treatment of LARC patients. These findings could be clinically relevant and support the use of mRNA levels when aiming to identify patients who respond to CRT therapy.
Sponsorship: C, CC and AB were supported by projects CTS2200 and PI-0710-2013 of Junta de Andalucía, TIN2013-41990-R of Programa Estatal I+D+i MINECO, and GREIB PYR_2010-02 and 2010_05 of University of Granada.
Publisher: Public Library of Science (PLOS)
Keywords: Cancer treatment
Gene expression
Microarrays
Rectal cancer
Gene amplification
Genetic networks
Messenger RNA
Surgical oncology
URI: http://hdl.handle.net/10481/34958
ISSN: 1932-6203
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Palma, P.; et al. Expression Profiling of Rectal Tumors Defines Response to Neoadjuvant Treatment Related Genes. Plos One, 9(11): e112189 (2014). [http://hdl.handle.net/10481/34958]
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