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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/34954

Title: Pine Bark and Green Tea Concentrated Extracts: Antioxidant Activity and Comprehensive Characterization of Bioactive Compounds by HPLC–ESI-QTOF-MS
Authors: Cádiz-Gurrea, María de la Luz
Fernández-Arroyo, Salvador
Segura Carretero, Antonio
Issue Date: 2014
Abstract: The consumption of polyphenols has frequently been associated with low incidence of degenerative diseases. Most of these natural antioxidants come from fruits, vegetables, spices, grains and herbs. For this reason, there has been increasing interest in identifying plant extract compounds. Polymeric tannins and monomeric flavonoids, such as catechin and epicatechin, in pine bark and green tea extracts could be responsible for the higher antioxidant activities of these extracts. The aim of the present study was to characterize the phenolic compounds in pine bark and green tea concentrated extracts using high-performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC–ESI-QTOF-MS). A total of 37 and 35 compounds from pine bark and green tea extracts, respectively, were identified as belonging to various structural classes, mainly flavan-3-ol and its derivatives (including procyanidins). The antioxidant capacity of both extracts was evaluated by three complementary antioxidant activity methods: Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC). Higher antioxidant activity values by each method were obtained. In addition, total polyphenol and flavan-3-ol contents, which were determined by Folin–Ciocalteu and vanillin assays, respectively, exhibited higher amounts of gallic acid and (+)-catechin equivalents.
Sponsorship: This work was supported by the project AGL2011-29857-C03-02 and AP2010-1551 (Spanish Ministry of Science and Innovation), as well as P09-CTS-4564, P10-FQM-6563 and P11-CTS-7625 (Andalusian Regional Government Council of Innovation and Science). The authors are also grateful to Instituto de Salud Carlos III for the Sara Borrell Grant (CD12/00672)
Publisher: MDPI
Keywords: Pine bark
Green tea
Polyphenols
Flavan-3-ols
Procyanidins
Antioxidant activity
HPLC–ESI-QTOF-MS
URI: http://hdl.handle.net/10481/34954
ISSN: 1422-0067
Citation: Cádiz-Gurrea, M.L.; Fernández-Arroyo, S.; Segura-Carretero, A. Pine Bark and Green Tea Concentrated Extracts: Antioxidant Activity and Comprehensive Characterization of Bioactive Compounds by HPLC–ESI-QTOF-MS. International Journal of Molecular Sciences, 15(11): 20382-20402 (2014). [http://hdl.handle.net/10481/34954]
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