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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/33899

Title: Nontemplated Nucleotide Additions Distinguish the Small RNA Composition in Cells from Exosomes
Authors: Koppers-Lalic, Danijela
Hackenberg, Michael
Bijnsdorp, Irene V.
Eijndhoven, Monique A. J. van
Sadek, Payman
Sie, Daud
Zini, Nicoletta
Middeldorp, Jaap M.
Ylstra, Bauke
Menezes, Renee X. de
Würdinger, Thomas
Meijer, Gerrit A.
Pegtel, D. Michiel
Issue Date: 2014
Abstract: Functional biomolecules, including small noncoding RNAs (ncRNAs), are released and transmitted between mammalian cells via extracellular vesicles (EVs), including endosome-derived exosomes. The small RNA composition in cells differs from exosomes, but underlying mechanisms have not been established. We generated small RNA profiles by RNA sequencing (RNA-seq) from a panel of human B cells and their secreted exosomes. A comprehensive bioinformatics and statistical analysis revealed nonrandomly distributed subsets of microRNA (miRNA) species between B cells and exosomes. Unexpectedly, 3′ end adenylated miRNAs are relatively enriched in cells, whereas 3′ end uridylated isoforms appear overrepresented in exosomes, as validated in naturally occurring EVs isolated from human urine samples. Collectively, our findings suggest that posttranscriptional modifications, notably 3′ end adenylation and uridylation, exert opposing effects that may contribute, at least in part, to direct ncRNA sorting into EVs.
Sponsorship: T.W. is supported by VIDI 91711366. D.M.P. is supported by personal Dutch Cancer Society research award (KWF-5510). This work was funded by AICR grant 11-0157 and NWO-VENI 91696087 awarded to D.M.P.
Publisher: Cell Press
Keywords: Biomolecules
Noncoding RNAs (ncRNAS)
B cells (Human)
Posttranscriptional modifications
URI: http://hdl.handle.net/10481/33899
ISSN: 2211-1247
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Koppers-Lalic, D.; et al. Nontemplated Nucleotide Additions Distinguish the Small RNA Composition in Cells from Exosomes. Cell Reports, 8: 1649-1658 (2014). [http://hdl.handle.net/10481/33899]
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