Characterization of the Exopolysaccharide Produced by Salipiger mucosus A3T, a Halophilic Species Belonging to the Alphaproteobacteria, Isolated on the Spanish Mediterranean Seaboard
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AuthorLlamas Company, Inmaculada; Mata Gómez, Juan Antonio; Tallón, Richard; Bressollier, Philippe; Urdaci, María C.; Quesada Arroquia, Emilia; Béjar Luque, María Victoria
ExopolysaccharidesSalipiger mucosusHalophilic bacteriaFucoseSulfates
Llamas, I.; et al. Characterization of the Exopolysaccharide Produced by Salipiger mucosus A3T, a Halophilic Species Belonging to the Alphaproteobacteria, Isolated on the Spanish Mediterranean Seaboard. Marine Drugs, 8(8): 2240-2251 (2010). [http://hdl.handle.net/10481/33536]
SponsorshipThis research was supported by grants from the Dirección General de Investigación Científica y Técnica (BOS2003-00498; CGL 2005-05947) and from the Plan Andaluz de Investigación, Spain.
We have studied the exopolysaccharide produced by the type strain of Salipiger mucosus, a species of halophilic, EPS-producing (exopolysaccharide-producing) bacterium belonging to the Alphaproteobacteria. The strain, isolated on the Mediterranean seaboard, produced a polysaccharide, mainly during its exponential growth phase but also to a lesser extent during the stationary phase. Culture parameters influenced bacterial growth and EPS production. Yield was always directly related to the quantity of biomass in the culture. The polymer is a heteropolysaccharide with a molecular mass of 250 kDa and its components are glucose (19.7%, w/w), mannose (34%, w/w), galactose (32.9%, w/w) and fucose (13.4%, w/w). Fucose and fucose-rich oligosaccharides have applications in the fields of medicine and cosmetics. The chemical or enzymatic hydrolysis of fucose-rich polysaccharides offers a new efficient way to process fucose. The exopolysaccharide in question produces a solution of very low viscosity that shows pseudoplastic behavior and emulsifying activity on several hydrophobic substrates. It also has a high capacity for binding cations and incorporating considerable quantities of sulfates, this latter feature being very unusual in bacterial polysaccharides.