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dc.contributor.authorReitz, Thomas
dc.contributor.authorRossberg, Andre
dc.contributor.authorBarkleit, Astrid
dc.contributor.authorSelenska-Pobell, Sonja
dc.contributor.authorLarbi Merroun, Mohamed
dc.date.accessioned2014-10-02T09:59:01Z
dc.date.available2014-10-02T09:59:01Z
dc.date.issued2014
dc.identifier.citationReitz, T.; et al. Decrease of U(VI) Immobilization Capability of the Facultative Anaerobic Strain Paenibacillus sp. JG-TB8 under Anoxic Conditions Due to Strongly Reduced Phosphatase Activity. Plos One, 9(8): e102447 (2014). [http://hdl.handle.net/10481/33318]es_ES
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10481/33318
dc.description.abstractInteractions of a facultative anaerobic bacterial isolate named Paenibacillus sp. JG-TB8 with U(VI) were studied under oxic and anoxic conditions in order to assess the influence of the oxygen-dependent cell metabolism on microbial uranium mobilization and immobilization. We demonstrated that aerobically and anaerobically grown cells of Paenibacillus sp. JG-TB8 accumulate uranium from aqueous solutions under acidic conditions (pH 2 to 6), under oxic and anoxic conditions. A combination of spectroscopic and microscopic methods revealed that the speciation of U(VI) associated with the cells of the strain depend on the pH as well as on the aeration conditions. At pH 2 and pH 3, uranium was exclusively bound by organic phosphate groups provided by cellular components, independently on the aeration conditions. At higher pH values, a part (pH 4.5) or the total amount (pH 6) of the dissolved uranium was precipitated under oxic conditions in a meta-autunite-like uranyl phosphate mineral phase without supplying an additional organic phosphate substrate. In contrast to that, under anoxic conditions no mineral formation was observed at pH 4.5 and pH 6, which was clearly assigned to decreased orthophosphate release by the cells. This in turn was caused by a suppression of the indigenous phosphatase activity of the strain. The results demonstrate that changes in the metabolism of facultative anaerobic microorganisms caused by the presence or absence of oxygen can decisively influence U(VI) biomineralization.es_ES
dc.description.sponsorshipThis work was partially funded by the grants CGL2009-09760 and CGL2012-36505 (Ministerio de Ciencia e Innovación, Spain).es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS)es_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 License
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subjectAbsorption spectroscopyes_ES
dc.subjectAnaerobic bacteria es_ES
dc.subjectLuminescence es_ES
dc.subjectOxygen es_ES
dc.subjectPhosphatases es_ES
dc.subjectPhosphateses_ES
dc.subjectPrecipitateses_ES
dc.subjectUraniumes_ES
dc.titleDecrease of U(VI) Immobilization Capability of the Facultative Anaerobic Strain Paenibacillus sp. JG-TB8 under Anoxic Conditions Due to Strongly Reduced Phosphatase Activityes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.1371/journal.pone.0102447


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