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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/32890

Title: Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality
Authors: Giampieri, Francesca
Álvarez-Suárez, José M.
Mazzoni, Luca
Forbes-Hernández, Tamara Y.
Gasparrini, Massimiliano
Gonzàlez-Paramàs, Ana M.
Santos-Buelga, Celestino
Quiles Morales, José Luis
Bompadre, Stefano
Mezzetti, Bruno
Battino, Maurizio
Issue Date: 2014
Abstract: Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and β-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging.
Publisher: MDPI
Keywords: Strawberry
Anthocyanin
ROS
Lipid peroxidation
DNA damage
Fibroblasts
URI: http://hdl.handle.net/10481/32890
ISSN: 1420-3049
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Giampieri, F.; et al. Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality. Molecules, 19(6): 7798-7816 (2014). [http://hdl.handle.net/10481/32890]
Appears in Collections:DFisiología - Artículos

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