Leishmanicidal activity of nine novel flavonoids from delphinim staphisagria
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AutorRamírez-Macias, Inmaculada; Marín Sánchez, Clotilde; Díaz, Jesús G.; Rosales Lombardo, María José; Gutiérrez-Sánchez, Ramón; Sánchez-Moreno, Manuel
Hindawi Publishing Corporation
In-vitro activityNatural productsEnergy metabolismCell deathTrypanosomatidsPromastigotesInfantumDrugs
Ramírez-Macias, I.; et al. Leishmanicidal activity of nine novel flavonoids from delphinim staphisagria. Scientific World Journal, 2012: 203646 (2012). [http://hdl.handle.net/10481/32044]
PatrocinadorThis work was financially supported by the Consolider Ingenio Project CSD2010-00065 (Spain).
Objectives. To evaluate the in vitro leishmanicidal activity of nine flavonoid derivatives from Delphinium staphisagria against L. infantum and L. braziliensis. Design and Methods. The in vitro activity of compounds 1–9 was assayed on extracellular promastigote and axenic amastigote forms and on intracellular amastigote forms of the parasites. Infectivity and cytotoxicity tests were carried on J774.2 macrophage cells using Glucantime as the reference drug. The mechanisms of action were analysed performing metabolite excretion and transmission electronic microscope ultrastructural alteration studies. Results. Nine flavonoids showed leishmanicidal activity against promastigote as well as amastigote forms of Leishmania infantum and L. braziliensis. These compounds were nontoxic to mammalian cells and were effective at similar concentrations up to or lower than that of the reference drug (Glucantime). The results showed that 2" -acetylpetiolaroside (compound 8) was clearly the most active. Conclusion. This study has demonstrated that flavonoid derivatives are active against L. infantum and L. braziliensis.