Animal bones char solubilization by gel-entrapped yarrowia lipolytica on glycerol-based media
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Hindawi Publishing Corporation
Soil plant systemYarowia lipolyticaEncapsulated cellsCitric acidPhosphateResiduesFungusOil
Vassileva, M.; et al. Animal bones char solubilization by gel-entrapped yarrowia lipolytica on glycerol-based media. Scientific World Journal, 2012: 907143 (2012). [http://hdl.handle.net/10481/32038]
PatrocinadorThis work was supported by Projects CTM2008-03524, CTM2011-027797 (Ministerio de Ciencia e Innovación, España), P09-RNM-5196 (Junta de Andalucía, Proyecto de Excelencia), and EU COST FA0905. N. Vassileva is grateful for the SABF PR2010-0422—Ministerio de Educacion, España.
Citric acid was produced with free and k-carrageenan-entrapped cells of the yeast Yarrowia lipolytica in single and repeated batch-shake-flask fermentations on glycerol-based media. Simultaneous solubilization of hydroxyapatite of animal bone origin (HABO) was tested in all experiments. The highest citric acid production by free yeast cells of 20.4 g/L and 18.7 g/L was reached after 96 h of fermentation in the absence and presence of 3 g/L HABO, respectively. The maximum values for the same parameter achieved by gel-entrapped cells in conditions of single batch and repeated-batch fermentation processes were 18.7 g/L and 28.1 g/L registered after 96 h and the 3d batch cycle, respectively. The highest citric acid productivity of 0.58 g L−1 h−1 was obtained with immobilized cells in repeated batch mode of fermentation when the added hydroxyapatite of 3 g/L was solubilized to 399 mg/L whereas the maximum efficiency of 89.0% was obtained with 1 g/L of HABO.