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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/31655

Title: Genomic profile of trinitrobencenesulfonic acid (TNBS)-induced colitis model (GSE9293)
Authors: Sánchez de Medina, Fermín
Martínez-Augustín, Olga
Montero Meléndez, Trinidad
Suárez García, Antonio
Zarzuelo Zurita, Antonio
Suárez, María Dolores
Llor, Xavier
Vieites Fernández, José M.
Gassull, Miguel
Issue Date: 1-Nov-2008
Abstract: Trinitrobenzenesulfonic acid (TNBS) rat colitis is one of the most widely used models of inflammatory bowel disease, a condition whose etiology and pathophysiology are incompletely understood. We have characterized the model at the genomic level following a longitudinal approach.
Description: [Organism] Rattus norvegicus
[Experiment type] Expression profiling by array
[Overall design] A single enema of 10mg of TNBS in 50% ethanol was administered to rats at day 0. Samples were recovered at days 2, 5, 7 and 14. According to inflammatory markers (myeloperoxidase activity, body weigh loss, colonic weigh/length ratio) we selected three replicates at each time point for the genimoc analysis, and 6 healthy control rats that received a saline enema. RNA was extracted from homogenized full-thickness colonic tissues in Trizol® reagent (Invitrogen) and purified with RNeasy affinity columns (Qiagen), according to manufacturer´s protocol. The microarray analysis was performed by Progenika Biopharma (Bilbao, Spain) on GeneChip® Rat Genome 230 2.0 Array (Affymetrix). All sample labeling (biotin), hybridization, staining and scanning procedures were carried out using Affimetrix, standard protocols (www.affymetrix.com). Normalization was carried out using Bioconductor sofware (affyPLM package).
[Platforms] GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
[Relations] BioProject PRJNA102933
1. GSM235052 Healthy control, replicate 1. [Characteristics] Sprague-Dawley female rats Tissue: full-thickness colon Rat received water
2. GSM235053 Healthy control, replicate 2. Characteristics Sprague-Dawley female rats Tissue:full-thickness colon Rat received water
3. GSM235055 Helathy control, replicate 3. [Characteristics] Sprague-Dawley female rats Tissue: full-thickness colon Rat received water
4.GSM235056 Healthy control, replicate 4. [Characteristics] Sprague-Dawley female rats Tissue: full-thickness colon Rat received water
5. GSM235057 Healthy control, replicate 5. [Characteristics] Sprague-Dawley female rats Tissue: full-thickness colon Rat received water
6. GSM235058 Healthy control, replicate 6. [Characteristics] Sprague-Dawley female rats Tissue: full-thickness colon Rat received water
7. GSM235552 TNBS day 2, replicate 1. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0
8. GSM235558 TNBS day 2, replicate 2. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0
9. GSM235559 TNBS day 2, replicate 3. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0
10. GSM235643 TNBS day 5, replicate 1. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0
11. GSM235745 TNBS day 5, replicate 2. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0
12. GSM235832 TNBS day 5, replicate 3. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0
13. GSM235872 TNBS day 7, replicate 1. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0
14. GSM235873 TNBS day 7, replicate 2. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0
15. GSM236126 TNBS day 7, replicate 3.[Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0
16. GSM236922 TNBS day 14, replicate 1. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0
17. GSM236924 TNBS day 14, replicate 2. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0
18. GSM236925 TNBS day 14, replicate 3. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0
Keywords: Animals
Chordates
Mammals
Nonhuman vertebrates
Rodents
Muridae
Rattus norvegicus
Dataset
URI: http://hdl.handle.net/10481/31655
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Appears in Collections:Información complementaria de las Investigaciones (Datos en Bruto)

Files in This Item:

File Description SizeFormat
GSM235052.xls1. GSM235052 Healthy control, replicate 12.06 MBMicrosoft ExcelView/Open
GSM235053.xls2. GSM235053 Healthy control, replicate 22.05 MBMicrosoft ExcelView/Open
GSM235055.xls3. GSM235055 Helathy control, replicate 32.05 MBMicrosoft ExcelView/Open
GSM235056.xls4. GSM235056 Healthy control, replicate 42.05 MBMicrosoft ExcelView/Open
GSM235057.xls5. GSM235057 Healthy control, replicate 52.05 MBMicrosoft ExcelView/Open
GSM235058.xls6. GSM235058 Healthy control, replicate 62.05 MBMicrosoft ExcelView/Open
GSM235552.xls7. GSM235552 TNBS day 2, replicate 12.05 MBMicrosoft ExcelView/Open
GSM235558.xls8. GSM235558 TNBS day 2, replicate 22.05 MBMicrosoft ExcelView/Open
GSM235559.xls9. GSM235559 TNBS day 2, replicate 32.05 MBMicrosoft ExcelView/Open
GSM235643.xls10. GSM235643 TNBS day 5, replicate 12.05 MBMicrosoft ExcelView/Open
GSM235745.xls11. GSM235745 TNBS day 5, replicate 22.05 MBMicrosoft ExcelView/Open
GSM235832.xls12. GSM235832 TNBS day 5, replicate 32.05 MBMicrosoft ExcelView/Open
GSM235872.xls13. GSM235872 TNBS day 7, replicate 12.05 MBMicrosoft ExcelView/Open
GSM235873.xls14. GSM235873 TNBS day 7, replicate 22.05 MBMicrosoft ExcelView/Open
GSM236126.xls15. GSM236126 TNBS day 7, replicate 32.05 MBMicrosoft ExcelView/Open
GSM236922.xls16. GSM236922 TNBS day 14, replicate 12.05 MBMicrosoft ExcelView/Open
GSM236924.xls17. GSM236924 TNBS day 14, replicate 22.05 MBMicrosoft ExcelView/Open
GSM236925.xls18. GSM236925 TNBS day 14, replicate 32.05 MBMicrosoft ExcelView/Open
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