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dc.contributor.authorAguilera García, Concepción María 
dc.contributor.authorTofe, Inés
dc.contributor.authorSuárez, Antonio
dc.contributor.authorGómez Llorente, Carolina 
dc.contributor.authorCañete, Ramón
dc.contributor.authorGil Hernández, Ángel 
dc.date.accessioned2014-05-08T13:02:01Z
dc.date.available2014-05-08T13:02:01Z
dc.date.issued2008-10-01
dc.identifier.otherGEO accession: GSE9624
dc.identifier.urihttp://hdl.handle.net/10481/31635
dc.description[Organism] Homo sapienses_ES
dc.description[Experiment type] Expression profiling by arrayes_ES
dc.description[Overall design] Adipose tissue and blood samples were obtained from 27 children, 14 obese (BMI adjusted for age and sex z score > 2) and 13 non obese undergoing appendix surgery. About 400 mg of adipose tissue was taken and immediately immersed in RNAlater solution and stored at -80°C for gene expression analysis. Informed consent was obtained from all patients after the nature of the study was explained, and the experimental design was approved, from an ethical and scientific standpoint, by the Hospital’s Ethical Committee responsible for research.es_ES
dc.description[Platforms (1)] GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Arrayes_ES
dc.description[Relations] BioProject PRJNA103477es_ES
dc.description1. GSM243215 Adipose tissue_423_control_rep1. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 10 year z score bmi: -1.45es_ES
dc.description2. GSM243216 Adipose tissue_425_control_rep2. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 7 year z score bmi: 0.49es_ES
dc.description3. GSM243217 Adipose tissue_426_control_rep3. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: F age: 8 year z score bmi: 0.12es_ES
dc.description4. GSM243218 Adipose tissue_427_control_rep4. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 10 year z score bmi: -1.81es_ES
dc.description5. GSM243219 Adipose tissue_428_control_rep5. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 7 year z score bmi: -0.64es_ES
dc.description6. GSM243220 Adipose tissue_429_control_rep6. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 7 year z score bmi: -0.36es_ES
dc.description7. GSM243225 Adipose tissue_418_obese_rep1. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 11 years z score bmi: 2es_ES
dc.description8. GSM243226 Adipose tissue_419_obese_rep2. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 9 years z score bmi: 4.25es_ES
dc.description9. GSM243227 Adipose tissue_420_obese_rep3. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 12 years z score bmi: 2.36es_ES
dc.description10. GSM243228 Adipose tissue_421_obese_rep4. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 9 years z score bmi: 4.25es_ES
dc.description11. GSM243275 Adipose tissue_422_obese_rep5. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 10 years z score bmi: 2.72es_ES
dc.description.abstractCharacterization of genes associated with adipose tissue is key to understanding the pathogenesis of obesity and developing treatments for this disorder. Differential gene expression in the adipose tissue has been described in adulthood but none studies have been developed on childhood. The purpose of this study was to compare gene expression in omental adipose tissue from obese prepubertal and normal weight children. We selected 5 obese (BMI adjusted for age and sex z score >2) and 6 normal weight children. RNA was extracted from omental adipose tissue biopsies and cRNA was hybridizated on the human genome U133 Plus 2.0 Arrays (Affymetrix®). Microarray experiments were performed for each sample, and selected group of gene expression values were confirmed with real-time RT-PCR in 10 obese and 10 normal weigth prepubertal children. 1276 genes were found to be differentially expressed at P<0.05. Of those differential genes, 201 were upregulated (Fc>2) and 42 were downregulated (Fc<-2). Genes involved in metabolic and signalling pathways were altered in childhood obesity. Keywords: disease state analysises_ES
dc.language.isoenges_ES
dc.publisherUniversidad de Granadaes_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 License
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subjectDataseten_US
dc.subjectAnimals es_ES
dc.subjectChordateses_ES
dc.subjectHumanses_ES
dc.subjectMammals es_ES
dc.subjectPrimates es_ES
dc.subjectVertebrates es_ES
dc.subjectHominidaees_ES
dc.subjectHomo sapienses_ES
dc.titleDifferential gene expression in omental adipose tissue from obese children (GSE9624)es_ES
dc.typedatasetes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.30827/Digibug.31635
dc.publication.year2008es_ES


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