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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/31635

Title: Differential gene expression in omental adipose tissue from obese children (GSE9624)
Authors: Aguilera García, Concepción
Tofe, Inés
Suárez, Antonio
Gómez Llorente, Carolina
Cañete, Ramón
Gil Hernández, Ángel
Issue Date: 1-Oct-2008
Abstract: Characterization of genes associated with adipose tissue is key to understanding the pathogenesis of obesity and developing treatments for this disorder. Differential gene expression in the adipose tissue has been described in adulthood but none studies have been developed on childhood. The purpose of this study was to compare gene expression in omental adipose tissue from obese prepubertal and normal weight children. We selected 5 obese (BMI adjusted for age and sex z score >2) and 6 normal weight children. RNA was extracted from omental adipose tissue biopsies and cRNA was hybridizated on the human genome U133 Plus 2.0 Arrays (Affymetrix®). Microarray experiments were performed for each sample, and selected group of gene expression values were confirmed with real-time RT-PCR in 10 obese and 10 normal weigth prepubertal children. 1276 genes were found to be differentially expressed at P<0.05. Of those differential genes, 201 were upregulated (Fc>2) and 42 were downregulated (Fc<-2). Genes involved in metabolic and signalling pathways were altered in childhood obesity. Keywords: disease state analysis
Description: [Organism] Homo sapiens
[Experiment type] Expression profiling by array
[Overall design] Adipose tissue and blood samples were obtained from 27 children, 14 obese (BMI adjusted for age and sex z score > 2) and 13 non obese undergoing appendix surgery. About 400 mg of adipose tissue was taken and immediately immersed in RNAlater solution and stored at -80°C for gene expression analysis. Informed consent was obtained from all patients after the nature of the study was explained, and the experimental design was approved, from an ethical and scientific standpoint, by the Hospital’s Ethical Committee responsible for research.
[Platforms (1)] GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
[Relations] BioProject PRJNA103477
1. GSM243215 Adipose tissue_423_control_rep1. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 10 year z score bmi: -1.45
2. GSM243216 Adipose tissue_425_control_rep2. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 7 year z score bmi: 0.49
3. GSM243217 Adipose tissue_426_control_rep3. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: F age: 8 year z score bmi: 0.12
4. GSM243218 Adipose tissue_427_control_rep4. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 10 year z score bmi: -1.81
5. GSM243219 Adipose tissue_428_control_rep5. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 7 year z score bmi: -0.64
6. GSM243220 Adipose tissue_429_control_rep6. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 7 year z score bmi: -0.36
7. GSM243225 Adipose tissue_418_obese_rep1. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 11 years z score bmi: 2
8. GSM243226 Adipose tissue_419_obese_rep2. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 9 years z score bmi: 4.25
9. GSM243227 Adipose tissue_420_obese_rep3. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 12 years z score bmi: 2.36
10. GSM243228 Adipose tissue_421_obese_rep4. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 9 years z score bmi: 4.25
11. GSM243275 Adipose tissue_422_obese_rep5. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 10 years z score bmi: 2.72
Keywords: Animals
Chordates
Humans
Mammals
Primates
Vertebrates
Hominidae
Homo sapiens
Dataset
URI: http://hdl.handle.net/10481/31635
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Appears in Collections:Información complementaria de las Investigaciones (Datos en Bruto)

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File Description SizeFormat
GSM243215.xlsAdipose tissue_423_control_rep13.48 MBMicrosoft ExcelView/Open
GSM243216.xlsAdipose tissue_425_control_rep23.47 MBMicrosoft ExcelView/Open
GSM243217.xlsAdipose tissue_426_control_rep33.48 MBMicrosoft ExcelView/Open
GSM243218.xlsAdipose tissue_427_control_rep43.48 MBMicrosoft ExcelView/Open
GSM243219.xlsAdipose tissue_428_control_rep53.47 MBMicrosoft ExcelView/Open
GSM243220.xlsAdipose tissue_429_control_rep63.45 MBMicrosoft ExcelView/Open
GSM243225.xlsAdipose tissue_418_obese_rep13.48 MBMicrosoft ExcelView/Open
GSM243226.xlsAdipose tissue_419_obese_rep23.48 MBMicrosoft ExcelView/Open
GSM243227.xlsAdipose tissue_420_obese_rep33.49 MBMicrosoft ExcelView/Open
GSM243228.xlsAdipose tissue_421_obese_rep43.48 MBMicrosoft ExcelView/Open
GSM243275.xlsAdipose tissue_422_obese_rep53.48 MBMicrosoft ExcelView/Open
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