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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/31187

Title: An Expressed Sequence Tag (EST)-enriched genetic map of turbot (Scophthalmus maximus): a useful framework for comparative genomics across model and farmed teleosts
Authors: Bouza, Carmen
Hermida, Miguel
Pardo, Belén G.
Vera, Manuel
Fernández, Carlos
Herrán Moreno, Roberto de la
Navajas-Pérez, Rafael
Álvarez-Dios, José Antonio
Gómez-Tato, Antonio
Martínez, Paulino
Issue Date: 2012
Abstract: [Background] The turbot (Scophthalmus maximus) is a relevant species in European aquaculture. The small turbot genome provides a source for genomics strategies to use in order to understand the genetic basis of productive traits, particularly those related to sex, growth and pathogen resistance. Genetic maps represent essential genomic screening tools allowing to localize quantitative trait loci (QTL) and to identify candidate genes through comparative mapping. This information is the backbone to develop marker-assisted selection (MAS) programs in aquaculture. Expressed sequenced tag (EST) resources have largely increased in turbot, thus supplying numerous type I markers suitable for extending the previous linkage map, which was mostly based on anonymous loci. The aim of this study was to construct a higher-resolution turbot genetic map using EST-linked markers, which will turn out to be useful for comparative mapping studies. [Results] A consensus gene-enriched genetic map of the turbot was constructed using 463 SNP and microsatellite markers in nine reference families. This map contains 438 markers, 180 EST-linked, clustered at 24 linkage groups. Linkage and comparative genomics evidences suggested additional linkage group fusions toward the consolidation of turbot map according to karyotype information. The linkage map showed a total length of 1402.7 cM with low average intermarker distance (3.7 cM; ~2 Mb). A global 1.6:1 female-to-male recombination frequency (RF) ratio was observed, although largely variable among linkage groups and chromosome regions. Comparative sequence analysis revealed large macrosyntenic patterns against model teleost genomes, significant hits decreasing from stickleback (54%) to zebrafish (20%). Comparative mapping supported particular chromosome rearrangements within Acanthopterygii and aided to assign unallocated markers to specific turbot linkage groups. [Conclusions] The new gene-enriched high-resolution turbot map represents a useful genomic tool for QTL identification, positional cloning strategies, and future genome assembling. This map showed large synteny conservation against model teleost genomes. Comparative genomics and data mining from landmarks will provide straightforward access to candidate genes, which will be the basis for genetic breeding programs and evolutionary studies in this species.
Sponsorship: This study was supported by the projects: Consolider Ingenio Aquagenomics (CSD200700002), Spanish Ministerio de Ciencia e Innovación (AGL2009-13273), and Xunta de Galicia local Government (09MMA011261PR). We are indebted to Lucía Insua, María Portela, Susana Sánchez, María López, Mónica Otero and Sonia Gómez for technical assistance. B.G. Pardo was supported by an Isidro Parga Pondal research fellowship from Xunta de Galicia (Spain).
Publisher: Biomed Central
Keywords: Turbot
Scopththalmus maximus
Pleuronectiformes
Genetic map
Recombination frequency
Comparative mapping
URI: http://hdl.handle.net/10481/31187
ISSN: 1471-2156
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Bouza, C.; et al. An Expressed Sequence Tag (EST)-enriched genetic map of turbot (Scophthalmus maximus): a useful framework for comparative genomics across model and farmed teleosts. BMC Genetics, 13: 54 (2012). [http://hdl.handle.net/10481/31187]
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