Universidad de Granada Digibug

Repositorio Institucional de la Universidad de Granada >
1.-Investigación >
Departamentos, Grupos de Investigación e Institutos >
Departamento de Histología >
DH - Artículos >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/30773

Title: Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells
Authors: Berdasco, María
Alcázar, Rubén
García-Ortiz, María Victoria
Ballestar, Esteban
Fernández, Agustín F.
Roldán-Arjona, Teresa
Tiburcio, Antonio F.
Altabella, Teresa
Buisine, Nicolas
Quesneville, Hadi
Baudry, Antoine
Lepiniec, Loïc
Alaminos Mingorance, Miguel
Rodríguez, Roberto
Lloyd, Alan
Colot, Vicent
Bender, Judith
Canal, María Jesús
Esteller, Manel
Fraga, Mario F.
Issue Date: 2008
Abstract: Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches to show that Arabidopsis cell suspensions and calluses specifically repress some genes as a result of promoter DNA hypermethylation. We found that promoters of the MAPK12, GSTU10 and BXL1 genes become hypermethylated in callus cells and that hypermethylation also affects the TTG1, GSTF5, SUVH8, fimbrin and CCD7 genes in cell suspensions. Promoter hypermethylation in undifferentiated cells was associated with histone hypoacetylation and primarily occurred at CpG sites. Accordingly, we found that the process specifically depends on MET1 and DRM2 methyltransferases, as demonstrated with DNA methyltransferase mutants. Our results suggest that promoter DNA methylation may be another important epigenetic mechanism for the establishment and/or maintenance of the undifferentiated state in plant cells.
Sponsorship: This work was supported by the Health (FIS01-04) (PI061267), Education and Science (I+D+I MCYT08-03, FU2004-02073/BMC and Consolider MEC09-05) Departments of the Spanish Government, the European Grant TRANSFOG LSHC-CT-2004-503438, and the Spanish Association Against Cancer (AECC). M.B. is funded by the Association Against Cancer (AECC).
Publisher: Public Library of Science (PLOS)
Keywords: Arabidopsis thaliana
Cell differentiation
Transposable elements
URI: http://hdl.handle.net/10481/30773
ISSN: 1932-6203
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Berdasco, M.; et al. Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells. Plos One, 3(10): e3306 (2008). [http://hdl.handle.net/10481/30773]
Appears in Collections:DH - Artículos

Files in This Item:

File Description SizeFormat
Berdasco_DNA.pdf673.17 kBAdobe PDFView/Open
Recommend this item

This item is licensed under a Creative Commons License
Creative Commons

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! OpenAire compliant DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard - Feedback

© Universidad de Granada