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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/30761

Title: Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering
Authors: Sánchez Quevedo, María del Carmen
Alaminos Mingorance, Miguel
Capitán Cañadas, Luis Miguel
Moreu Burgos, Gerardo
Garzón Bello, Ingrid Johanna
Crespo Ferrer, Pascual Vicente
Campos Muñoz, Antonio
Issue Date: 2007
Abstract: Reconstruction of large oral mucosa defects is often challenging, since the shortage of healthy oral mucosa to replace the excised tissues is very common. In this context, tissue engineering techniques may provide a source of autologous tissues available for transplant in these patients. In this work, we developed a new model of artificial oral mucosa generated by tissue engineering using a fibrin-agarose scaffold. For that purpose, we generated primary cultures of human oral mucosa fibroblasts and keratinocytes from small biopsies of normal oral mucosa using enzymatic treatments. Then we determined the viability of the cultured cells by electron probe quantitative X-ray microanalysis, and we demonstrated that most of the cells in the primary cultures were alive and had high K/Na ratios. Once cell viability was determined, we used the cultured fibroblasts and keratinocytes to develop an artificial oral mucosa construct by using a fibrin-agarose extracellular matrix and a sequential culture technique using porous culture inserts. Histological analysis of the artificial tissues showed high similarities with normal oral mucosa controls. The epithelium of the oral substitutes had several layers, with desmosomes and apical microvilli and microplicae. Both the controls and the oral mucosa substitutes showed high suprabasal expression of cytokeratin 13 and low expression of cytokeratin 10. All these results suggest that our model of oral mucosa using fibrin-agarose scaffolds show several similarities with native human oral mucosa.
Sponsorship: This work was supported by the grants FIS 03/0141 and FIS 04/1306 from the Spanish National Ministry of Health (Instituto de Salud Carlos III) and by CM 2005/011 from Junta de Andalucía.
Publisher: Universidad de Murcia
Keywords: Fibrin-agarose
Constructs
Tissue engineering
URI: http://hdl.handle.net/10481/30761
ISSN: 0213-3911
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Sánchez Quevedo, M.C.; et al. Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering. Histology and Histopathology, 22(6): 631-640 (2007). [http://hdl.handle.net/10481/30761]
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