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dc.contributor.authorOrtega-González, Mercedes
dc.contributor.authorSánchez De Medina López-Huertas, Fermín 
dc.contributor.authorMolina Santiago, Carlos Alberto
dc.contributor.authorLópez-Posadas, Rocío
dc.contributor.authorPacheco, Daniel
dc.contributor.authorKrell, Tino
dc.contributor.authorMartínez-Augustín, Olga
dc.contributor.authorDaddaoua, Abdelali 
dc.date.accessioned2014-03-06T08:42:35Z
dc.date.available2014-03-06T08:42:35Z
dc.date.issued2014
dc.identifier.citationOrtega-González, M.; et al. Fructooligosacharides Reduce Pseudomonas aeruginosa PAO1 Pathogenicity through Distinct Mechanisms. Plos One, 9(1): e85772 (2014). [http://hdl.handle.net/10481/30691]es_ES
dc.identifier.issn1932-6203
dc.identifier.otherdoi: 10.1371/journal.pone.0085772
dc.identifier.urihttp://hdl.handle.net/10481/30691
dc.description.abstractPseudomonas aeruginosa is ubiquitously present in the environment and acts as an opportunistic pathogen on humans, animals and plants. We report here the effects of the prebiotic polysaccharide inulin and its hydrolysed form FOS on this bacterium. FOS was found to inhibit bacterial growth of strain PAO1, while inulin did not affect growth rate or yield in a significant manner. Inulin stimulated biofilm formation, whereas a dramatic reduction of the biofilm formation was observed in the presence of FOS. Similar opposing effects were observed for bacterial motility, where FOS inhibited the swarming and twitching behaviour whereas inulin caused its stimulation. In co-cultures with eukaryotic cells (macrophages) FOS and, to a lesser extent, inulin reduced the secretion of the inflammatory cytokines IL-6, IL-10 and TNF- a . Western blot experiments indicated that the effects mediated by FOS in macrophages are associated with a decreased activation of the NF- k B pathway. Since FOS and inulin stimulate pathway activation in the absence of bacteria, the FOS mediated effect is likely to be of indirect nature, such as via a reduction of bacterial virulence. Further, this modulatory effect is observed also with the highly virulent ptxS mutated strain. Co-culture experiments of P. aeruginosa with IEC18 eukaryotic cells showed that FOS reduces the concentration of the major virulence factor, exotoxin A, suggesting that this is a possible mechanism for the reduction of pathogenicity. The potential of these compounds as components of antibacterial and anti-inflammatory cocktails is discussed.es_ES
dc.description.sponsorshipThe authors acknowledge financial support from FEDER funds and Fondo Social Europeo through grants from the Spanish Ministry of Economy and Competitiveness (grants SAF2011-22922, SAF2011-22812) the Andalusian regional government Junta de Andalucía (grant CVI-7335) and the Centre of Networked Biomedical Research on Hepatic and Digestive Diseases (CIBERehd) which is funded by the Carlos III Health Institute and the Ramón Areces Foundation, Spain.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS)es_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Licensees_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es_ES
dc.subjectToll-like receptorses_ES
dc.subjectBiofilm formationes_ES
dc.subjectTwitcing motilityes_ES
dc.subjectEpithelial-cellses_ES
dc.subjectSecretion systemes_ES
dc.subjectLung infectiones_ES
dc.subjectAntimicrobial activityes_ES
dc.subjectProtein secretiones_ES
dc.subjectGene-expressiones_ES
dc.titleFructooligosacharides Reduce Pseudomonas aeruginosa PAO1 Pathogenicity through Distinct Mechanismses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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