Ex vivo microbial leakage after using different final irrigation regimens with chlorhexidine
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AuthorNavarro Escobar, Esther; Baca García, Pilar; González Rodríguez, María Paloma; Arias-Moliz, Teresa; Ruiz-Linares, Matilde; Ferrer Luque, Carmen María
Universidade de Sao Paulo. Faculdade de Odontologia de Bauru
Bacterial leakageCetrimideChiorthexidineEnterococcus faecalisIrrigation
Navarro Escobar, E.; et al. Ex vivo microbial leakage after using different final irrigation regimens with chlorhexidine. Journal of Applied Oral Science, 21(1): 74-79 (2013). [http://hdl.handle.net/10481/29883]
Objective To assess the influence of final irrigation protocols with chlorhexidine in the coronal leakage of Enterococcus faecalis in filled root canals. Material and Methods Seventy single-root canals from extracted teeth were prepared using ProTaper instruments. The irrigation protocol accomplished an alternating irrigation with 5 mL of 2.5% sodium hypochlorite (NaOCI) and 17% EDTA between each file. The teeth were randomly divided into four experimental groups (n=15) according to the final irrigation regimen: group 1, without final irrigation; group 2, irrigation with 10 mL 2.0% chlorhexidine (CHX); group 3, with a final application of EC40™; and group 4, irrigation with the combination (1:1) of 0.2% CHX + 0.1% cetrimide (CTR). All the teeth were mounted in a two-chamber apparatus and the coronal access was exposed to E. faecalis. The presence of turbidity in the BHI broth over a period of 180 days was observed. The Friedman test was used for statistical analysis. Results EC40™ varnish showed the least leakage at 180 days, and was statistically similar to 2% CHX. No significant differences were observed between the group without final irrigation and the 2% CHX group or 0.2% CHX + 0.1% CTR. Conclusions In this ex vivo study, EC40™ showed the longest delayed coronal leakage of E. faecalis, although without significant differences from 2% CHX.