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dc.contributor.authorPalma Carazo, Pablo
dc.contributor.authorCuadros Celorrio, Marta Eugenia 
dc.contributor.authorConde Muíño, Raquel
dc.contributor.authorOlmedo, Carmen
dc.contributor.authorCano Gutiérrez, Carlos 
dc.contributor.authorSegura-Jiménez, Inmaculada
dc.contributor.authorBlanco Morón, Armando 
dc.contributor.authorBueno Laraño, Pablo
dc.contributor.authorFerrón Orihuela, José Antonio
dc.contributor.authorMedina Vico, Pedro Pablo 
dc.date.accessioned2013-12-19T13:50:30Z
dc.date.available2013-12-19T13:50:30Z
dc.date.issued2013
dc.identifier.citationPalma, P.; et al. Microarray profiling of mononuclear peripheral blood cells identifies novle candidate genes related to chemoradiation response in rectal cancer. Plos One, 8(9): e74034 (2013). [http://hdl.handle.net/10481/29712]es_ES
dc.identifier.issn1932-6203
dc.identifier.otherdoi: 10.1371/journal.pone.0074034
dc.identifier.urihttp://hdl.handle.net/10481/29712
dc.description.abstractPreoperative chemoradiation significantly improves oncological outcome in locally advanced rectal cancer. However there is no effective method of predicting tumor response to chemoradiation in these patients. Peripheral blood mononuclear cells have emerged recently as pathology markers of cancer and other diseases, making possible their use as therapy predictors. Furthermore, the importance of the immune response in radiosensivity of solid organs led us to hypothesized that microarray gene expression profiling of peripheral blood mononuclear cells could identify patients with response to chemoradiation in rectal cancer. Thirty five 35 patients with locally advanced rectal cancer were recruited initially to perform the study. Peripheral blood samples were obtained before neaodjuvant treatment. RNA was extracted and purified to obtain cDNA and cRNA for hybridization of microarrays included in Human WG CodeLink bioarrays. Quantitative real time PCR was used to validate microarray experiment data. Results were correlated with pathological response, according to Mandard´s criteria and final UICC Stage (patients with tumor regression grade 1–2 and downstaging being defined as responders and patients with grade 3–5 and no downstaging as non-responders). Twenty seven out of 35 patients were finally included in the study. We performed a multiple t-test using Significance Analysis of Microarrays, to find those genes differing significantly in expression, between responders (n = 11) and non-responders (n = 16) to CRT. The differently expressed genes were: BC 035656.1, CIR, PRDM2, CAPG, FALZ, HLA-DPB2, NUPL2, and ZFP36. The measurement of FALZ (p = 0.029) gene expression level determined by qRT-PCR, showed statistically significant differences between the two groups. Gene expression profiling reveals novel genes in peripheral blood samples of mononuclear cells that could predict responders and non-responders to chemoradiation in patients with locally advanced rectal cancer. Moreover, our investigation added further evidence to the importance of mononuclear cells’ mediated response in the neoadjuvant treatment of rectal cancer.es_ES
dc.description.sponsorshipThis investigation was supported by the Fundación Investigación Biomédica Mutua Madrileña. MC, CC and AB were supported by projects P08-TIC-4299 and CTS2200 of Junta de Andalucía, TIN2009-13489 of DGICT, Madrid, and GREIB PYR_2010-02 and 2010_05 of University of Granada.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS)es_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Licensees_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es_ES
dc.subjectCancer treatmentes_ES
dc.subjectComplementary DNAes_ES
dc.subjectGene expressiones_ES
dc.subjectImmune responsees_ES
dc.subjectMicroarrayses_ES
dc.subjectRNA extractiones_ES
dc.subjectRectal canceres_ES
dc.subjectSurgical pathologyes_ES
dc.titleMicroarray profiling of mononuclear peripheral blood cells identifies novle candidate genes related to chemoradiation response in rectal canceres_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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