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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/29701

Title: Effects of adult exposure to bisphenol A on genes involved in the physiopathology of rat prefrontal cortex
Authors: Castro, Beatriz
Sánchez Medina, María del Pilar
Torres de Pinedo, Jesús Manuel
Ortega Sánchez, Esperanza
Issue Date: 2013
Abstract: Several neurological and behavioral dysfunctions have been reported in animals exposed to bisphenol A (BPA). However, little is known about the impact of adult exposure to BPA on brain physiopathology. Here, we focused on prefrontal cortex (PFC) of rats, because it is an important area for cognitive control, complex behaviors and is altered in many psychopathologies. Gamma-aminobutyric acid (GABA) and serotonin (5-HT) systems are essential for PFC function. Therefore, we examined the effects of adult exposure to BPA on 5α-Reductase (5α-R) and cytochrome P450 aromatase (P450arom), enzymes that synthesize GABAA receptor modulators, and tryptophan hydroxylase (Tph), the rate-limiting enzyme in 5-HT biosynthesis. To gain better understanding of BPA’s action in the adult PFC, 84 genes involved in neurotoxicity were also analysed. Adult male and female rats were subcutaneously injected for 4 days with 50 µg/kg/day, the current reference safe dose for BPA. mRNA and protein levels of 5α-R, P450arom and Tph were quantified by real-time RT-PCR and Western blot. Genes linked to neurotoxicity were analyzed by PCR-Array technology. Adult exposure to BPA increased both P450arom and Tph2 expression in PFC of male and female, but decreased 5α-R1 expression in female. Moreover, we identified 17 genes related to PFC functions such as synaptic plasticity and memory, as potential targets of BPA. Our results provided new insights on the molecular mechanisms underlying BPA action in the physiopathology of PFC, but also raise the question about the safety of short-term exposure to it in the adulthood.
Sponsorship: This research was supported by grants from Ministerio de Ciencia e Innovación (BFU2008-05340) and by the Junta de Andalucía (CTS202-Endocronología y Metabolismo).
Publisher: Public Library of Science (PLOS)
Keywords: Biosynthesis
Gamma-aminobutyric acid
Isozymes
Messenger RNA
Polymerase chain reaction
Prefrontal cortex
Synaptic plasticity
Tryptophan
URI: http://hdl.handle.net/10481/29701
ISSN: 1932-6203
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Castro, B.; et al. Effects of adult exposure to bisphenol A on genes involved in the physiopathology of rat prefrontal cortex. Plos One, 8(9): e73584 (2013). [http://hdl.handle.net/10481/29701]
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